Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 267648..268279 Replicon chromosome
Accession NZ_AP021914
Organism Escherichia coli strain WP2-W18-CRE-03

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H7Q98_RS01175 Protein ID WP_001259388.1
Coordinates 267648..267923 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H7Q98_RS01180 Protein ID WP_000593555.1
Coordinates 267920..268279 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7Q98_RS01160 262652..263719 + 1068 WP_000361485.1 HlyD family secretion protein -
H7Q98_RS01165 263716..266451 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
H7Q98_RS01170 266451..267575 + 1125 WP_001314210.1 ABC transporter permease -
H7Q98_RS01175 267648..267923 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H7Q98_RS01180 267920..268279 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H7Q98_RS01185 268399..268800 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H7Q98_RS01190 268806..269612 - 807 WP_124783472.1 nickel import ATP-binding protein NikE -
H7Q98_RS01195 269609..270373 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H7Q98_RS01200 270373..271206 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
H7Q98_RS01205 271203..272147 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T33715 WP_001259388.1 NZ_AP021914:267648-267923 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T33715 NZ_AP021914:267648-267923 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT33715 WP_000593555.1 NZ_AP021914:267920-268279 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT33715 NZ_AP021914:267920-268279 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References