Detailed information of TA system TA033699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1766206..1766796
• Replicon: chromosome
• Accession: NZ_AP021909
• Organism: Klebsiella pneumoniae strain WP2-S17-ESBL-02

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: H7R06_RS08535
• Protein ID: WP_023341911.1
• Coordinates: 1766464..1766796 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: H7R06_RS08530
• Protein ID: WP_000288812.1
• Coordinates: 1766206..1766463 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R06_RS08510	1762541..1764289	+	1749	WP_032418698.1	hypothetical protein	-
H7R06_RS08515	1764343..1764924	+	582	WP_099184326.1	hypothetical protein	-
H7R06_RS08520	1765207..1765668	+	462	WP_004213450.1	hypothetical protein	-
H7R06_RS08525	1765665..1765871	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
H7R06_RS08530	1766206..1766463	+	258	WP_000288812.1	antitoxin	Antitoxin
H7R06_RS08535	1766464..1766796	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H7R06_RS08545	1767118..1768554	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
H7R06_RS08555	1768920..1770374	-	1455	WP_004148975.1	AMP nucleosidase	-
H7R06_RS08560	1770504..1770749	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T33699 WP_023341911.1 NZ_AP021909:1766464-1766796 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T33699 NZ_AP021909:1766464-1766796 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT33699 WP_000288812.1 NZ_AP021909:1766206-1766463 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT33699 NZ_AP021909:1766206-1766463 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0