Detailed information of TA system TA033695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 268054..268702
• Replicon: chromosome
• Accession: NZ_AP021908
• Organism: Stenotrophomonas maltophilia strain WP1-W18-CRE-01

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2FJ07
• Locus tag: H7X90_RS01200
• Protein ID: WP_012478870.1
• Coordinates: 268054..268341 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: H7X90_RS01205
• Protein ID: WP_012478871.1
• Coordinates: 268400..268702 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7X90_RS01170	263314..264282	+	969	WP_049460450.1	bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA	-
H7X90_RS01175	264279..265010	+	732	WP_183153720.1	type III pantothenate kinase	-
H7X90_RS01180	265020..265868	+	849	WP_065183740.1	SPOR domain-containing protein	-
H7X90_RS01190	266069..267193	-	1125	WP_012478868.1	hypothetical protein	-
H7X90_RS01195	267221..267820	-	600	WP_012478869.1	hypothetical protein	-
H7X90_RS01200	268054..268341	+	288	WP_012478870.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7X90_RS01205	268400..268702	+	303	WP_012478871.1	putative addiction module antidote protein	Antitoxin
H7X90_RS01210	268760..269083	-	324	WP_012478872.1	hypothetical protein	-
H7X90_RS01215	269163..269570	-	408	WP_044569408.1	hypothetical protein	-
H7X90_RS01220	270164..270934	+	771	WP_012478874.1	DUF3011 domain-containing protein	-
H7X90_RS01225	270994..271938	+	945	WP_012478875.1	ankyrin repeat domain-containing protein	-
H7X90_RS01230	272043..272963	+	921	WP_005407709.1	arginase	-
H7X90_RS01235	273124..273261	+	138	WP_005407710.1	entericidin A/B family lipoprotein	-
H7X90_RS01240	273468..273689	+	222	WP_004153772.1	CsbD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-95)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10802.42 Da
• Isoelectric Point: 10.8940

>T33695 WP_012478870.1 NZ_AP021908:268054-268341 [Stenotrophomonas maltophilia]
MKIQRTRQFATWIDALKDVTARARILARIGRLAEGHPGDHRYLGDGISELRIDAGPGYRLYYTQRGRQLLILLVGGDKSS
QQRDIEKAREIARAL

Nucleotide

• Length: 288 bp

>T33695 NZ_AP021908:268054-268341 [Stenotrophomonas maltophilia]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGACGTAACCGCACGCGCTCGCATCCTGGC
CCGTATCGGTCGGCTCGCTGAAGGCCATCCGGGTGATCACCGCTATCTGGGGGACGGTATTTCCGAACTGCGTATCGATG
CAGGGCCGGGCTACCGCCTGTACTACACCCAGCGCGGCAGGCAGTTGCTGATTCTTTTGGTCGGAGGTGACAAAAGCTCA
CAGCAACGCGATATCGAAAAGGCCAGGGAGATTGCACGTGCCCTGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10738.32 Da
• Isoelectric Point: 8.0343

>AT33695 WP_012478871.1 NZ_AP021908:268400-268702 [Stenotrophomonas maltophilia]
MATKKKVELKSFDVAEHLRTPEEMAAYLDACIEESDGDSAFIAKALGDIARAQGMSKVARAAGLSRESLYRALSGERSPD
FATILKVTRALGVRLHASAV

Nucleotide

• Length: 303 bp

>AT33695 NZ_AP021908:268400-268702 [Stenotrophomonas maltophilia]
ATGGCCACTAAGAAAAAAGTCGAACTCAAATCATTTGATGTGGCCGAACACCTTCGGACACCCGAAGAAATGGCAGCCTA
TCTCGACGCCTGCATCGAGGAAAGCGACGGCGATTCCGCGTTCATCGCCAAGGCGCTCGGCGACATCGCGCGCGCTCAGG
GTATGAGCAAGGTTGCGCGCGCGGCGGGCCTGTCGCGCGAGAGTCTTTACCGCGCACTGTCCGGCGAACGCAGCCCCGAT
TTCGCCACCATCCTGAAGGTGACCCGTGCCTTGGGTGTGCGCCTGCATGCCAGTGCCGTGTGA

Structures

Toxin

• AlphaFold DB: A0A854E218