Detailed information of TA system TA033690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 7005881..7006521
• Replicon: chromosome
• Accession: NZ_AP021904
• Organism: Pseudomonas sp. Ost2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KI243_RS31165
• Protein ID: WP_017125396.1
• Coordinates: 7006108..7006521 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A7Y7Y0G0
• Locus tag: KI243_RS31160
• Protein ID: WP_042935145.1
• Coordinates: 7005881..7006111 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI243_RS31135 (PHLH7_61370)	7001651..7002778	+	1128	WP_212810263.1	S8 family peptidase	-
KI243_RS31140 (PHLH7_61380)	7002843..7004018	-	1176	WP_177128265.1	tetratricopeptide repeat protein	-
KI243_RS31145 (PHLH7_61390)	7004250..7004987	+	738	WP_212810264.1	hypothetical protein	-
KI243_RS31150 (PHLH7_61400)	7005035..7005205	+	171	WP_085983767.1	YhfG family protein	-
KI243_RS33030	7005277..7005390	+	114	Protein_6163	Fic family protein	-
KI243_RS31160 (PHLH7_61420)	7005881..7006111	+	231	WP_042935145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI243_RS31165	7006108..7006521	+	414	WP_017125396.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI243_RS31170 (PHLH7_61440)	7006675..7007043	+	369	WP_177087131.1	DUF1493 family protein	-
KI243_RS31175 (PHLH7_61450)	7007091..7008638	-	1548	WP_017125398.1	phosphoenolpyruvate carboxykinase	-
KI243_RS31180 (PHLH7_61460)	7008818..7009720	-	903	WP_017125399.1	Hsp33 family molecular chaperone HslO	-
KI243_RS31185 (PHLH7_61470)	7009870..7010274	-	405	WP_177087133.1	S4 domain-containing protein	-
KI243_RS31190 (PHLH7_61480)	7010488..7010970	+	483	WP_212811265.1	ATP-dependent zinc protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15429.74 Da
• Isoelectric Point: 7.7754

>T33690 WP_017125396.1 NZ_AP021904:7006108-7006521 [Pseudomonas sp. Ost2]
VTTYMLDTCICSFIMRERPESVLKRLAAEVGRNNRIVISAITYSEMRYGQIGKKASSRHKIMIDEFVKRLDAILPWDQNA
VDATVEVRRQLTDIGSLIGGNDTAIAGHAIATGCKLITNNLREFSRVTGLEYEDWVH

Nucleotide

• Length: 414 bp

>T33690 NZ_AP021904:7006108-7006521 [Pseudomonas sp. Ost2]
GTGACCACCTATATGCTCGATACCTGTATTTGCTCGTTCATCATGCGTGAACGCCCCGAATCTGTATTGAAGCGATTGGC
AGCAGAAGTGGGTCGAAATAACCGGATCGTAATCTCTGCGATCACTTACTCAGAAATGCGCTACGGACAGATCGGCAAAA
AAGCTTCCTCCAGGCACAAAATAATGATCGATGAGTTTGTGAAGCGCCTGGATGCGATCCTGCCGTGGGATCAGAACGCC
GTGGACGCGACAGTCGAAGTGAGACGCCAACTGACGGATATCGGCTCGCTCATTGGCGGTAATGACACGGCCATCGCGGG
CCACGCCATAGCCACGGGATGCAAGCTGATCACCAACAATCTACGTGAGTTCAGCCGTGTTACAGGGCTCGAATACGAAG
ACTGGGTTCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8500.61 Da
• Isoelectric Point: 4.5045

>AT33690 WP_042935145.1 NZ_AP021904:7005881-7006111 [Pseudomonas sp. Ost2]
MRTVSIFKNGKNQAIRLPNDMAYEGVGELEITRNGDVITLRPVRPSWQSLAELPAADADFLQERPDIIGDEGRFSL

Nucleotide

• Length: 231 bp

>AT33690 NZ_AP021904:7005881-7006111 [Pseudomonas sp. Ost2]
ATGCGCACAGTCTCAATTTTCAAGAACGGCAAAAATCAGGCGATACGCCTGCCAAATGACATGGCTTATGAAGGCGTAGG
AGAACTGGAGATCACGCGCAATGGCGATGTGATCACCCTGCGCCCTGTGCGCCCCAGCTGGCAATCGCTGGCAGAGCTAC
CTGCGGCTGATGCTGACTTTCTACAAGAACGCCCGGACATCATCGGCGATGAAGGCAGGTTCAGTCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Y7Y0G0