Detailed information of TA system TA033688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5142911..5143521
• Replicon: chromosome
• Accession: NZ_AP021904
• Organism: Pseudomonas sp. Ost2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KI243_RS22490
• Protein ID: WP_017129567.1
• Coordinates: 5142911..5143093 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7Y8CB20
• Locus tag: KI243_RS22495
• Protein ID: WP_017129568.1
• Coordinates: 5143117..5143521 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI243_RS22475 (PHLH7_44370)	5139445..5140647	-	1203	WP_212809928.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KI243_RS22480 (PHLH7_44380)	5140663..5142132	-	1470	WP_103032663.1	efflux transporter outer membrane subunit	-
KI243_RS22485 (PHLH7_44390)	5142122..5142604	-	483	WP_017129566.1	MarR family transcriptional regulator	-
KI243_RS22490 (PHLH7_44400)	5142911..5143093	+	183	WP_017129567.1	type II toxin-antitoxin system HicA family toxin	Toxin
KI243_RS22495 (PHLH7_44410)	5143117..5143521	+	405	WP_017129568.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KI243_RS22500 (PHLH7_44420)	5143518..5144132	-	615	WP_017129569.1	tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase MiaE	-
KI243_RS22505 (PHLH7_44430)	5144372..5145232	+	861	WP_017129570.1	universal stress protein	-
KI243_RS22510 (PHLH7_44440)	5145263..5145733	-	471	WP_017129571.1	DUF1289 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6710.90 Da
• Isoelectric Point: 11.6500

>T33688 WP_017129567.1 NZ_AP021904:5142911-5143093 [Pseudomonas sp. Ost2]
VKSRDLIAELEAAGWMLQRIKGSHHVFVHSTNPHSIPVPHPKKDLPLGTIRSIRKRAGLL

Nucleotide

• Length: 183 bp

>T33688 NZ_AP021904:5142911-5143093 [Pseudomonas sp. Ost2]
GTGAAGAGCAGGGATTTGATCGCCGAACTGGAGGCCGCCGGCTGGATGCTGCAACGAATCAAGGGTAGTCATCATGTGTT
CGTTCACAGCACCAACCCTCATTCGATTCCCGTGCCACATCCCAAGAAGGATCTGCCACTCGGAACTATCAGAAGCATCA
GAAAACGGGCGGGCCTGCTATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14793.80 Da
• Isoelectric Point: 4.4915

>AT33688 WP_017129568.1 NZ_AP021904:5143117-5143521 [Pseudomonas sp. Ost2]
MQYPIAIEWGDEHTAIGIQVPDIPGAVTAGDTFEEAYNAAIEVAHIMLEEIASDGQPIPMPSSSLSHRKNPDFADMGWGM
LELDISPYLGKTEKVNVTLPGFVIQRIDRYVRDHQIKSRSSFLADAALEKLVRA

Nucleotide

• Length: 405 bp

>AT33688 NZ_AP021904:5143117-5143521 [Pseudomonas sp. Ost2]
ATGCAATATCCCATCGCCATTGAATGGGGTGATGAACACACCGCCATTGGCATCCAGGTTCCGGATATTCCCGGCGCCGT
GACTGCCGGCGACACCTTCGAGGAGGCTTATAATGCCGCCATCGAGGTCGCCCATATAATGCTCGAGGAAATCGCGTCGG
ACGGACAACCGATCCCCATGCCCTCCTCGTCGCTTTCGCACCGGAAGAACCCCGACTTCGCCGACATGGGCTGGGGCATG
CTGGAGCTGGATATATCGCCCTATCTGGGCAAGACCGAGAAGGTCAACGTCACCCTTCCCGGTTTCGTGATCCAGCGCAT
CGACCGTTATGTACGCGATCACCAGATCAAGAGCCGATCGTCGTTCCTGGCCGATGCGGCGCTGGAAAAACTGGTTCGTG
CCTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Y8CB20