Detailed information of TA system TA033685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 4187446..4188035
• Replicon: chromosome
• Accession: NZ_AP021904
• Organism: Pseudomonas sp. Ost2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KI243_RS18355
• Protein ID: WP_017126106.1
• Coordinates: 4187446..4187697 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KI243_RS18360
• Protein ID: WP_212809636.1
• Coordinates: 4187694..4188035 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI243_RS18335 (PHLH7_36200)	4183206..4183946	+	741	WP_017126102.1	response regulator	-
KI243_RS18340 (PHLH7_36220)	4183943..4185255	+	1313	Protein_3637	HAMP domain-containing sensor histidine kinase	-
KI243_RS18345 (PHLH7_36230)	4185368..4185766	+	399	WP_017126104.1	RidA family protein	-
KI243_RS18350 (PHLH7_36240)	4185870..4187243	+	1374	WP_017126105.1	nucleotide 5'-monophosphate nucleosidase PpnN	-
KI243_RS18355 (PHLH7_36250)	4187446..4187697	+	252	WP_017126106.1	type II toxin-antitoxin system HicA family toxin	Toxin
KI243_RS18360 (PHLH7_36260)	4187694..4188035	+	342	WP_212809636.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KI243_RS18365 (PHLH7_36270)	4188037..4188672	-	636	WP_177083157.1	DNA-3-methyladenine glycosylase I	-
KI243_RS18370 (PHLH7_36280)	4188802..4189590	-	789	WP_017126109.1	porin family protein	-
KI243_RS18375 (PHLH7_36290)	4189740..4190294	-	555	WP_017126110.1	DUF3455 domain-containing protein	-
KI243_RS18380 (PHLH7_36300)	4190398..4191162	-	765	WP_212809638.1	trans-aconitate 2-methyltransferase	-
KI243_RS18385 (PHLH7_36310)	4191264..4192172	+	909	WP_212809640.1	LysR family transcriptional regulator	-
KI243_RS18390 (PHLH7_36320)	4192292..4192666	-	375	WP_177083159.1	tautomerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-74)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9428.93 Da
• Isoelectric Point: 10.8537

>T33685 WP_017126106.1 NZ_AP021904:4187446-4187697 [Pseudomonas sp. Ost2]
VSRNEKLLAKLLNPWMAFTWPELVTLLGHFGYRRLEGSGSRVKFDNGDPLAAISLHKPHPGNELRTYIRRQVIDHLRSGG
LIP

Nucleotide

• Length: 252 bp

>T33685 NZ_AP021904:4187446-4187697 [Pseudomonas sp. Ost2]
GTGTCCAGGAATGAAAAGCTGCTCGCAAAACTGCTCAACCCATGGATGGCCTTCACATGGCCGGAACTGGTCACGCTGCT
GGGCCATTTCGGCTACCGCCGGCTTGAAGGTTCAGGCAGCCGGGTCAAGTTCGACAACGGCGATCCGCTTGCAGCGATCA
GCCTGCATAAACCTCATCCAGGCAATGAGCTGAGAACCTATATCAGGCGTCAGGTGATCGACCATCTACGCTCAGGAGGA
CTGATTCCGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12721.40 Da
• Isoelectric Point: 6.4728

>AT33685 WP_212809636.1 NZ_AP021904:4187694-4188035 [Pseudomonas sp. Ost2]
VTNQMHYKGYFGSIEASVEDNCLFGKLQFIRALVSYEGQTVAELETAFKEAVEDYLDNCQRLKQSPEVPCKGSFNVRVGH
DLHLAAALSATRQKLTLNDLTRQALSEYLLRHA

Nucleotide

• Length: 342 bp

>AT33685 NZ_AP021904:4187694-4188035 [Pseudomonas sp. Ost2]
GTGACCAATCAGATGCACTACAAGGGCTATTTCGGCTCCATCGAAGCCAGCGTCGAGGACAACTGCCTGTTCGGCAAACT
TCAGTTCATCCGGGCGCTGGTGAGCTATGAGGGGCAGACCGTGGCGGAGCTGGAAACAGCCTTCAAGGAGGCGGTGGAGG
ACTATCTCGACAACTGCCAGCGCCTCAAGCAGTCTCCCGAAGTACCGTGCAAGGGTTCGTTCAACGTCCGTGTCGGCCAT
GACCTGCACCTGGCCGCCGCCCTCAGCGCCACGCGCCAGAAACTGACATTGAACGACCTGACCCGCCAGGCACTGAGCGA
ATACCTGCTGCGCCACGCCTGA