Detailed information of TA system TA033684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 2039119..2039632
• Replicon: chromosome
• Accession: NZ_AP021904
• Organism: Pseudomonas sp. Ost2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KI243_RS09100
• Protein ID: WP_178991782.1
• Coordinates: 2039119..2039403 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KI243_RS09105
• Protein ID: WP_177057445.1
• Coordinates: 2039393..2039632 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI243_RS32835	2035984..2036287	-	304	Protein_1791	bifunctional DNA primase/helicase	-
KI243_RS09090 (PHLH7_17870)	2036363..2037121	+	759	Protein_1792	hypothetical protein	-
KI243_RS09095 (PHLH7_17880)	2037235..2038644	+	1410	WP_244861886.1	hypothetical protein	-
KI243_RS09100	2039119..2039403	-	285	WP_178991782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI243_RS09105 (PHLH7_17890)	2039393..2039632	-	240	WP_177057445.1	ribbon-helix-helix protein, CopG family	Antitoxin
KI243_RS09110 (PHLH7_17900)	2039818..2040297	-	480	WP_212810708.1	Bro-N domain-containing protein	-
KI243_RS09120 (PHLH7_17910)	2040956..2041426	+	471	WP_212810710.1	DUF2442 domain-containing protein	-
KI243_RS09125 (PHLH7_17920)	2041437..2042846	-	1410	WP_017123942.1	DUF2252 domain-containing protein	-
KI243_RS09130 (PHLH7_17930)	2043071..2043538	+	468	WP_042934484.1	anti-virulence regulator CigR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10995.75 Da
• Isoelectric Point: 11.1493

>T33684 WP_178991782.1 NZ_AP021904:c2039403-2039119 [Pseudomonas sp. Ost2]
MRVEWLKTALRNLDDAAAYIAQDNPQAAQAFMLEVRASVQRLAQFPAMGREGRIPGTREWPLPDLPYLIPYRIRNGRLQV
LRVFHTRRLPPDQW

Nucleotide

• Length: 285 bp

>T33684 NZ_AP021904:c2039403-2039119 [Pseudomonas sp. Ost2]
ATGCGCGTTGAATGGCTGAAAACCGCACTCAGGAATCTGGATGACGCAGCTGCCTATATCGCGCAAGACAATCCACAAGC
AGCTCAGGCCTTCATGCTTGAGGTGCGCGCCAGCGTCCAGCGGCTAGCGCAGTTTCCCGCTATGGGCCGTGAAGGACGAA
TTCCAGGAACACGAGAATGGCCTCTTCCCGACCTGCCTTATCTGATTCCATACCGTATTCGTAACGGTCGTCTACAAGTG
CTGCGCGTCTTCCATACTCGGCGACTACCGCCTGACCAATGGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8805.87 Da
• Isoelectric Point: 4.2584

>AT33684 WP_177057445.1 NZ_AP021904:c2039632-2039393 [Pseudomonas sp. Ost2]
MSVMSLRLPDEMAETLANLAKATGRSKSFLAIDALRDYLAREAWQIAEIQRAIEEADAGDFATDEEVEATFRKWGADAR

Nucleotide

• Length: 240 bp

>AT33684 NZ_AP021904:c2039632-2039393 [Pseudomonas sp. Ost2]
ATGTCCGTTATGTCCCTACGTCTCCCCGACGAGATGGCTGAGACCCTCGCCAACCTGGCAAAAGCTACTGGCCGTAGTAA
ATCCTTCCTCGCCATCGATGCCCTACGGGACTACCTGGCCCGTGAAGCCTGGCAAATCGCCGAAATTCAGCGCGCTATAG
AAGAGGCTGATGCGGGAGACTTCGCCACGGACGAGGAAGTTGAAGCCACCTTCCGGAAATGGGGTGCCGATGCGCGTTGA