Detailed information of TA system TA033681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2421819..2422299
• Replicon: chromosome
• Accession: NZ_AP021903
• Organism: Pseudomonas sp. Seg1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KI231_RS10745
• Protein ID: WP_082341417.1
• Coordinates: 2421819..2422103 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4V6Q6W5
• Locus tag: KI231_RS10750
• Protein ID: WP_034152589.1
• Coordinates: 2422093..2422299 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI231_RS10715 (PHLH6_21160)	2417111..2417485	+	375	WP_064588154.1	type II secretion system protein	-
KI231_RS10720 (PHLH6_21170)	2417460..2417999	+	540	WP_213028196.1	type II secretion system GspH family protein	-
KI231_RS10725 (PHLH6_21180)	2417996..2418388	+	393	WP_213028197.1	curli production assembly/transport protein CsgE	-
KI231_RS10730 (PHLH6_21190)	2418385..2418816	+	432	WP_064120104.1	curli assembly protein CsgF	-
KI231_RS10735 (PHLH6_21200)	2418846..2419706	+	861	WP_083368634.1	CsgG/HfaB family protein	-
KI231_RS10740	2420972..2421342	+	371	Protein_2115	DUF6124 family protein	-
KI231_RS29960 (PHLH6_21220)	2421524..2421742	+	219	WP_034152590.1	hypothetical protein	-
KI231_RS10745	2421819..2422103	-	285	WP_082341417.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI231_RS10750 (PHLH6_21240)	2422093..2422299	-	207	WP_034152589.1	hypothetical protein	Antitoxin
KI231_RS10755 (PHLH6_21250)	2422570..2424021	-	1452	WP_213028198.1	curlin	-
KI231_RS10760 (PHLH6_21260)	2424050..2424514	-	465	WP_053119098.1	curlin	-
KI231_RS10765 (PHLH6_21270)	2424586..2426826	-	2241	WP_213028199.1	Ig-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10875.70 Da
• Isoelectric Point: 10.6630

>T33681 WP_082341417.1 NZ_AP021903:c2422103-2421819 [Pseudomonas sp. Seg1]
MQIKWRPKARVELSKILKYIGERNFVAATALHKSVVKTTSALCWHPHLYRKGRSPGTREIVVSPNYLVVYQVADHIEIVS
ILHARQEYPRRDPG

Nucleotide

• Length: 285 bp

>T33681 NZ_AP021903:c2422103-2421819 [Pseudomonas sp. Seg1]
ATGCAGATTAAGTGGCGACCCAAGGCCAGGGTCGAACTCTCGAAAATCCTTAAGTACATTGGCGAGCGAAATTTTGTAGC
GGCGACTGCACTTCACAAGTCCGTCGTAAAAACTACATCTGCGCTTTGTTGGCATCCACACCTGTATCGCAAAGGCCGCT
CTCCCGGCACTCGAGAGATTGTCGTAAGCCCGAACTATCTCGTCGTTTATCAAGTGGCGGACCACATTGAGATTGTGAGC
ATCTTGCATGCGCGTCAGGAGTACCCTCGACGCGACCCCGGTTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7672.54 Da
• Isoelectric Point: 3.8068

>AT33681 WP_034152589.1 NZ_AP021903:c2422299-2422093 [Pseudomonas sp. Seg1]
MIDPSLIDPLCPVVSDLDPEEQAASYDRWFRAEVQASLDDPRPSIPHEQVMEEINALMESMRKSADAD

Nucleotide

• Length: 207 bp

>AT33681 NZ_AP021903:c2422299-2422093 [Pseudomonas sp. Seg1]
ATGATCGACCCGTCTTTGATCGACCCACTGTGCCCGGTTGTTTCGGATCTTGATCCCGAGGAGCAGGCTGCAAGTTATGA
CCGTTGGTTTCGGGCCGAAGTACAGGCCTCTCTCGATGATCCGCGTCCGAGTATTCCCCATGAGCAGGTGATGGAGGAAA
TAAATGCCTTGATGGAATCAATGCGCAAAAGCGCTGATGCAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A4V6Q6W5