Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 359806..360440 Replicon chromosome
Accession NZ_AP021902
Organism Pseudomonas sp. Cab53

Toxin (Protein)


Gene name vapC Uniprot ID A0A0D0PFI3
Locus tag KI241_RS01535 Protein ID WP_042728141.1
Coordinates 359806..360210 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0G3G959
Locus tag KI241_RS01540 Protein ID WP_047736177.1
Coordinates 360210..360440 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI241_RS01510 (PHLH5_02980) 355124..356356 - 1233 WP_212803283.1 methyltransferase -
KI241_RS01515 (PHLH5_02990) 356337..357026 - 690 WP_123579377.1 ABC transporter permease -
KI241_RS01520 (PHLH5_03000) 357023..357718 - 696 WP_212803284.1 ABC transporter permease -
KI241_RS01525 (PHLH5_03010) 357797..358552 - 756 WP_053148690.1 ABC transporter substrate-binding protein -
KI241_RS01530 (PHLH5_03020) 358566..359339 - 774 WP_042728139.1 ABC transporter ATP-binding protein -
KI241_RS01535 (PHLH5_03030) 359806..360210 - 405 WP_042728141.1 tRNA(fMet)-specific endonuclease VapC Toxin
KI241_RS01540 (PHLH5_03040) 360210..360440 - 231 WP_047736177.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KI241_RS01545 (PHLH5_03050) 360530..360976 - 447 WP_212803285.1 hypothetical protein -
KI241_RS01550 (PHLH5_03060) 361016..363250 - 2235 WP_212803286.1 ATP-binding protein -
KI241_RS01555 (PHLH5_03070) 363247..363852 - 606 WP_042728145.1 biliverdin-producing heme oxygenase -
KI241_RS01560 (PHLH5_03080) 363926..364984 - 1059 WP_047736174.1 PDDEXK nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 323485..360440 36955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14886.45 Da        Isoelectric Point: 7.8031

>T33674 WP_042728141.1 NZ_AP021902:c360210-359806 [Pseudomonas sp. Cab53]
MIKFMLDTNVCIFTIKNKPQIVREAFNRHHGQLCISAVTLMELIYGAEKSSAPEKNLGVIEGFCARLEVLPFGYDAAAHT
GMIRAELAKIGRPIGPYDQMIAGHARSLGLIVVTNNLKEFERVPGLRIEDWTGM

Download         Length: 405 bp

>T33674 NZ_AP021902:c360210-359806 [Pseudomonas sp. Cab53]
ATGATCAAGTTCATGCTCGATACCAACGTCTGTATTTTCACCATCAAGAACAAGCCCCAGATCGTCAGAGAAGCTTTCAA
TCGTCATCACGGCCAGCTCTGCATCAGCGCCGTCACACTGATGGAGTTGATCTACGGCGCAGAAAAGTCATCCGCCCCAG
AGAAAAATCTCGGCGTCATCGAAGGGTTCTGTGCCCGACTTGAGGTTTTACCTTTCGGTTATGACGCCGCAGCCCATACC
GGCATGATCCGGGCAGAGCTGGCAAAGATTGGCAGGCCAATCGGCCCCTACGACCAGATGATTGCCGGCCATGCCCGCTC
CCTCGGGTTGATCGTAGTCACCAATAATCTGAAAGAATTCGAAAGAGTCCCCGGACTGCGCATCGAGGATTGGACAGGGA
TGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8560.57 Da        Isoelectric Point: 4.3719

>AT33674 WP_047736177.1 NZ_AP021902:c360440-360210 [Pseudomonas sp. Cab53]
MEQTTLFMSNRSQAVRLPKAVAMPSDVKRVDVIAIGRTRIITPAGEAWDSWFDGDNATADFMAEREQPADQERESF

Download         Length: 231 bp

>AT33674 NZ_AP021902:c360440-360210 [Pseudomonas sp. Cab53]
ATGGAACAAACCACCCTTTTCATGAGCAACCGCAGCCAAGCGGTCCGGCTACCCAAGGCAGTGGCGATGCCAAGCGACGT
AAAGCGTGTAGACGTGATCGCCATAGGCAGAACGCGCATTATCACGCCGGCTGGCGAAGCCTGGGATAGCTGGTTCGATG
GCGATAACGCCACGGCAGACTTCATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGTGAGTCTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D0PFI3

Antitoxin

Source ID Structure
AlphaFold DB A0A0G3G959

References