Detailed information of TA system TA033670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4658979..4659583
• Replicon: chromosome
• Accession: NZ_AP021900
• Organism: Pseudomonas sp. St386

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: KI235_RS20485
• Protein ID: WP_038858735.1
• Coordinates: 4658979..4659272 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1G4U731
• Locus tag: KI235_RS20490
• Protein ID: WP_038858737.1
• Coordinates: 4659275..4659583 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI235_RS20450 (PHLH3_40980)	4654362..4655219	+	858	WP_013693815.1	AraC family transcriptional regulator	-
KI235_RS20455 (PHLH3_40990)	4655249..4655464	+	216	WP_080560991.1	DUF6434 domain-containing protein	-
KI235_RS20460 (PHLH3_41000)	4655784..4655984	-	201	WP_013693816.1	hypothetical protein	-
KI235_RS30500 (PHLH3_41010)	4656090..4656515	-	426	WP_003203795.1	hypothetical protein	-
KI235_RS20470 (PHLH3_41020)	4656769..4656987	-	219	WP_100264119.1	hypothetical protein	-
KI235_RS20475 (PHLH3_41030)	4657049..4657594	-	546	WP_043042784.1	DUF4410 domain-containing protein	-
KI235_RS20480 (PHLH3_41040)	4658286..4658711	+	426	WP_233839793.1	hypothetical protein	-
KI235_RS20485 (PHLH3_41050)	4658979..4659272	+	294	WP_038858735.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI235_RS20490 (PHLH3_41060)	4659275..4659583	+	309	WP_038858737.1	putative addiction module antidote protein	Antitoxin
KI235_RS20495 (PHLH3_41070)	4660269..4660784	-	516	WP_233839794.1	hypothetical protein	-
KI235_RS20500 (PHLH3_41080)	4661265..4662134	+	870	WP_100264122.1	AraC family transcriptional regulator	-
KI235_RS20505	4662398..4662721	-	324	WP_100264123.1	DUF2388 domain-containing protein	-
KI235_RS20515 (PHLH3_41090)	4663113..4664396	+	1284	WP_027912980.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4645312..4662134	16822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10633.20 Da
• Isoelectric Point: 10.8582

>T33670 WP_038858735.1 NZ_AP021900:4658979-4659272 [Pseudomonas sp. St386]
MIYRIQQTQTFTAWHSSVRDLRAKNAIARRIDRAAAGNLGDVKPVGDGISELRVDVGAGYRDYFTMRGGLVIVLLAGGDK
SSQSADIRRAKKLAKEI

Nucleotide

• Length: 294 bp

>T33670 NZ_AP021900:4658979-4659272 [Pseudomonas sp. St386]
ATGATTTACCGCATACAGCAAACGCAGACGTTCACTGCTTGGCATTCATCGGTCCGTGATCTGCGAGCAAAAAACGCTAT
TGCCCGCCGCATTGATCGCGCCGCAGCAGGTAACCTGGGCGATGTGAAACCTGTCGGTGATGGTATTTCAGAGTTGCGTG
TGGATGTAGGCGCTGGCTACCGGGACTATTTCACCATGCGCGGTGGATTGGTCATCGTGCTACTGGCGGGTGGTGACAAG
TCTTCACAGAGTGCTGATATCCGGCGAGCGAAGAAATTGGCAAAGGAGATTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10928.34 Da
• Isoelectric Point: 5.9574

>AT33670 WP_038858737.1 NZ_AP021900:4659275-4659583 [Pseudomonas sp. St386]
MSQHLTTFDMAALLDSDEAISEYLSQVLADGDSEEFLRAIGYVAKARGMAQIAKDSGMGRESLYKAFAPGSKPRFDTVLK
VIHALGIDLHAQPGHAANHSPA

Nucleotide

• Length: 309 bp

>AT33670 NZ_AP021900:4659275-4659583 [Pseudomonas sp. St386]
ATGAGCCAACACCTGACGACATTTGACATGGCGGCACTACTCGATAGCGACGAAGCCATCAGTGAATACCTTTCCCAAGT
GCTGGCTGATGGTGATAGCGAGGAGTTTCTCCGAGCGATCGGCTATGTCGCCAAGGCCCGCGGAATGGCGCAGATCGCTA
AGGACTCTGGCATGGGGCGAGAAAGCTTGTACAAGGCGTTCGCACCCGGATCAAAGCCGAGATTTGATACCGTACTCAAG
GTCATTCATGCGCTAGGCATTGATCTTCATGCGCAGCCTGGGCATGCCGCTAATCATTCACCTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1G4U731