Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4084457..4085075 Replicon chromosome
Accession NZ_AP021896
Organism Escherichia coli strain 2018-02-2CC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HRR25_RS19445 Protein ID WP_001291435.1
Coordinates 4084457..4084675 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HRR25_RS19450 Protein ID WP_000344800.1
Coordinates 4084701..4085075 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR25_RS19410 4079746..4080318 + 573 WP_000779831.1 YbaY family lipoprotein -
HRR25_RS19415 4080349..4080660 - 312 WP_000409911.1 MGMT family protein -
HRR25_RS19425 4081039..4081392 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HRR25_RS19430 4081434..4082984 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HRR25_RS19435 4083148..4083618 - 471 WP_000136192.1 YlaC family protein -
HRR25_RS19440 4083734..4084285 - 552 WP_000102564.1 maltose O-acetyltransferase -
HRR25_RS19445 4084457..4084675 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HRR25_RS19450 4084701..4085075 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HRR25_RS19455 4085621..4088770 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HRR25_RS19460 4088793..4089986 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33669 WP_001291435.1 NZ_AP021896:c4084675-4084457 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33669 NZ_AP021896:c4084675-4084457 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33669 WP_000344800.1 NZ_AP021896:c4085075-4084701 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33669 NZ_AP021896:c4085075-4084701 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References