Detailed information of TA system TA033667

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3314543..3315378
• Replicon: chromosome
• Accession: NZ_AP021896
• Organism: Escherichia coli strain 2018-02-2CC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: HRR25_RS15805
• Protein ID: WP_000854821.1
• Coordinates: 3314543..3314920 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HRR25_RS15810
• Protein ID: WP_032216526.1
• Coordinates: 3315010..3315378 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR25_RS15785	3310910..3312448	-	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-
HRR25_RS15790	3313197..3314039	-	843	WP_172410720.1	DUF4942 domain-containing protein	-
HRR25_RS15795	3314124..3314321	-	198	WP_114141299.1	DUF957 domain-containing protein	-
HRR25_RS15800	3314397..3314546	-	150	Protein_3074	hypothetical protein	-
HRR25_RS15805	3314543..3314920	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HRR25_RS15810	3315010..3315378	-	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRR25_RS15815	3315397..3316041	-	645	Protein_3077	antitoxin of toxin-antitoxin stability system	-
HRR25_RS15820	3316060..3316281	-	222	WP_001601167.1	DUF987 domain-containing protein	-
HRR25_RS15825	3316350..3316826	-	477	WP_001186724.1	RadC family protein	-
HRR25_RS15830	3316842..3317327	-	486	WP_032216512.1	antirestriction protein	-
HRR25_RS15835	3317419..3318237	-	819	WP_053902307.1	DUF945 domain-containing protein	-
HRR25_RS15840	3318336..3318569	-	234	WP_032289278.1	DUF905 family protein	-
HRR25_RS15845	3318575..3319252	-	678	WP_032223228.1	hypothetical protein	-
HRR25_RS15850	3319371..3320255	-	885	WP_032216515.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3303422..3338861	35439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T33667 WP_000854821.1 NZ_AP021896:c3314920-3314543 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T33667 NZ_AP021896:c3314920-3314543 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT33667 WP_032216526.1 NZ_AP021896:c3315378-3315010 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT33667 NZ_AP021896:c3315378-3315010 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCTCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGTCGACCGCGCCGGCATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54