Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 541005..541643 Replicon chromosome
Accession NZ_AP021896
Organism Escherichia coli strain 2018-02-2CC

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HRR25_RS02700 Protein ID WP_000813794.1
Coordinates 541005..541181 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HRR25_RS02705 Protein ID WP_001270286.1
Coordinates 541227..541643 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR25_RS02680 536624..537838 - 1215 WP_172410564.1 BenE family transporter YdcO -
HRR25_RS02685 537891..538427 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HRR25_RS02690 538500..540461 + 1962 WP_172410565.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HRR25_RS02695 540553..540783 - 231 WP_000494244.1 YncJ family protein -
HRR25_RS02700 541005..541181 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HRR25_RS02705 541227..541643 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HRR25_RS02710 541722..543128 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HRR25_RS02715 543373..544518 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HRR25_RS02720 544536..545549 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HRR25_RS02725 545550..546491 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T33652 WP_000813794.1 NZ_AP021896:541005-541181 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T33652 NZ_AP021896:541005-541181 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT33652 WP_001270286.1 NZ_AP021896:541227-541643 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT33652 NZ_AP021896:541227-541643 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References