Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2560169..2560695 Replicon chromosome
Accession NZ_AP021893
Organism Escherichia coli strain 2018-06-4CC

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HRR20_RS12230 Protein ID WP_000323025.1
Coordinates 2560169..2560456 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HRR20_RS12235 Protein ID WP_000534858.1
Coordinates 2560456..2560695 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR20_RS12195 2555559..2555774 - 216 WP_000839565.1 phage lysis protein EssD -
HRR20_RS12200 2556026..2556400 - 375 WP_001348108.1 hypothetical protein -
HRR20_RS12205 2556572..2557000 - 429 WP_000506936.1 cell envelope integrity protein TolA -
HRR20_RS12210 2558045..2558587 - 543 WP_152922190.1 DUF1133 family protein -
HRR20_RS12215 2558584..2558874 - 291 WP_000247763.1 DUF1364 domain-containing protein -
HRR20_RS12220 2558874..2559473 - 600 WP_025670565.1 DUF1367 family protein -
HRR20_RS12225 2559942..2560097 - 156 WP_000813269.1 type I toxin-antitoxin system toxin HokD -
HRR20_RS12230 2560169..2560456 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HRR20_RS12235 2560456..2560695 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HRR20_RS12240 2560720..2561025 + 306 WP_172408818.1 hypothetical protein -
HRR20_RS12245 2561228..2561560 + 333 WP_001349760.1 FlxA-like family protein -
HRR20_RS12250 2561997..2563337 - 1341 WP_000589013.1 ISNCY family transposase -
HRR20_RS12260 2564134..2565333 + 1200 WP_001117227.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2525917..2588509 62592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T33605 WP_000323025.1 NZ_AP021893:c2560456-2560169 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T33605 NZ_AP021893:c2560456-2560169 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT33605 WP_000534858.1 NZ_AP021893:c2560695-2560456 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT33605 NZ_AP021893:c2560695-2560456 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References