Detailed information of TA system TA033564

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3560900..3561579
• Replicon: chromosome
• Accession: NZ_AP021891
• Organism: Escherichia coli strain 2017.01.04CC

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: VEGS_RS17205
• Protein ID: WP_000854680.1
• Coordinates: 3561238..3561579 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: VEGS_RS17200
• Protein ID: WP_000070396.1
• Coordinates: 3560900..3561217 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VEGS_RS17155	3556338..3557159	+	822	WP_023565123.1	DUF945 domain-containing protein	-
VEGS_RS17160	3557376..3558077	+	702	WP_047649085.1	WYL domain-containing protein	-
VEGS_RS17165	3558118..3558354	+	237	WP_001144031.1	hypothetical protein	-
VEGS_RS17170	3558354..3558797	+	444	WP_000649865.1	hypothetical protein	-
VEGS_RS17175	3558820..3559287	+	468	WP_001385283.1	hypothetical protein	-
VEGS_RS17180	3559364..3559603	+	240	Protein_3360	DUF905 domain-containing protein	-
VEGS_RS17185	3559701..3560159	+	459	WP_000211838.1	antirestriction protein	-
VEGS_RS17190	3560175..3560651	+	477	WP_000811693.1	RadC family protein	-
VEGS_RS17195	3560660..3560881	+	222	WP_124038440.1	DUF987 domain-containing protein	-
VEGS_RS17200	3560900..3561217	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
VEGS_RS17205	3561238..3561579	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
VEGS_RS17210	3562123..3563400	+	1278	WP_001269622.1	tyrosine-type recombinase/integrase	-
VEGS_RS17215	3563482..3564195	-	714	WP_000179449.1	hypothetical protein	-
VEGS_RS17220	3564230..3566068	-	1839	WP_097477492.1	hypothetical protein	-
VEGS_RS17225	3566083..3566286	-	204	WP_000452041.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3559364..3609983	50619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T33564 WP_000854680.1 NZ_AP021891:3561238-3561579 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T33564 NZ_AP021891:3561238-3561579 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT33564 WP_000070396.1 NZ_AP021891:3560900-3561217 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT33564 NZ_AP021891:3560900-3561217 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7