Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3390114..3390732 Replicon chromosome
Accession NZ_AP021891
Organism Escherichia coli strain 2017.01.04CC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag VEGS_RS16350 Protein ID WP_001291435.1
Coordinates 3390514..3390732 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag VEGS_RS16345 Protein ID WP_000344800.1
Coordinates 3390114..3390488 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VEGS_RS16335 3385203..3386396 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
VEGS_RS16340 3386419..3389568 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
VEGS_RS16345 3390114..3390488 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
VEGS_RS16350 3390514..3390732 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
VEGS_RS16355 3390904..3391455 + 552 WP_000102564.1 maltose O-acetyltransferase -
VEGS_RS16360 3391571..3392041 + 471 WP_000136192.1 YlaC family protein -
VEGS_RS16365 3392205..3393755 + 1551 WP_063081929.1 cyclic-guanylate-specific phosphodiesterase PdeB -
VEGS_RS16370 3393797..3394150 - 354 WP_000878140.1 DUF1428 domain-containing protein -
VEGS_RS16380 3394529..3394840 + 312 WP_000409911.1 MGMT family protein -
VEGS_RS16385 3394871..3395443 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33563 WP_001291435.1 NZ_AP021891:3390514-3390732 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33563 NZ_AP021891:3390514-3390732 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33563 WP_000344800.1 NZ_AP021891:3390114-3390488 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33563 NZ_AP021891:3390114-3390488 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References