Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3311422..3312047 Replicon chromosome
Accession NZ_AP021890
Organism Escherichia coli strain 2017.15.01CC

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HRR22_RS15800 Protein ID WP_000911329.1
Coordinates 3311422..3311820 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HRR22_RS15805 Protein ID WP_000450524.1
Coordinates 3311820..3312047 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR22_RS15780 3307300..3307500 + 201 WP_000383836.1 YpfN family protein -
HRR22_RS15785 3307610..3308308 - 699 WP_000679823.1 esterase -
HRR22_RS15790 3308382..3310397 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
HRR22_RS15795 3310412..3311275 - 864 WP_001267498.1 neutral zinc metallopeptidase -
HRR22_RS15800 3311422..3311820 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HRR22_RS15805 3311820..3312047 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HRR22_RS15810 3312202..3312915 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HRR22_RS15815 3313128..3314162 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HRR22_RS15820 3314179..3315057 - 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
HRR22_RS15825 3315203..3315775 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HRR22_RS15830 3315775..3316245 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T33539 WP_000911329.1 NZ_AP021890:c3311820-3311422 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T33539 NZ_AP021890:c3311820-3311422 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT33539 WP_000450524.1 NZ_AP021890:c3312047-3311820 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT33539 NZ_AP021890:c3312047-3311820 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References