Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1086142..1086760 Replicon chromosome
Accession NZ_AP021890
Organism Escherichia coli strain 2017.15.01CC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HRR22_RS05045 Protein ID WP_001291435.1
Coordinates 1086142..1086360 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HRR22_RS05050 Protein ID WP_000344800.1
Coordinates 1086386..1086760 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR22_RS05010 1081432..1082004 + 573 WP_000779831.1 YbaY family lipoprotein -
HRR22_RS05015 1082035..1082346 - 312 WP_000409911.1 MGMT family protein -
HRR22_RS05025 1082725..1083078 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HRR22_RS05030 1083120..1084670 - 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HRR22_RS05035 1084834..1085304 - 471 WP_000136192.1 YlaC family protein -
HRR22_RS05040 1085420..1085971 - 552 WP_000102578.1 maltose O-acetyltransferase -
HRR22_RS05045 1086142..1086360 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HRR22_RS05050 1086386..1086760 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HRR22_RS05055 1087306..1090455 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HRR22_RS05060 1090478..1091671 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33524 WP_001291435.1 NZ_AP021890:c1086360-1086142 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33524 NZ_AP021890:c1086360-1086142 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33524 WP_000344800.1 NZ_AP021890:c1086760-1086386 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33524 NZ_AP021890:c1086760-1086386 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References