Detailed information of TA system TA033522

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 889137..889971
• Replicon: chromosome
• Accession: NZ_AP021890
• Organism: Escherichia coli strain 2017.15.01CC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X7CAN1
• Locus tag: HRR22_RS04135
• Protein ID: WP_039025883.1
• Coordinates: 889594..889971 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2X5LBZ2
• Locus tag: HRR22_RS04130
• Protein ID: WP_039025882.1
• Coordinates: 889137..889505 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR22_RS04105	886253..887071	+	819	WP_001234702.1	DUF945 domain-containing protein	-
HRR22_RS04110	887162..887647	+	486	WP_063122055.1	antirestriction protein	-
HRR22_RS04115	887662..888138	+	477	WP_001186742.1	RadC family protein	-
HRR22_RS04120	888207..888428	+	222	WP_000692312.1	DUF987 domain-containing protein	-
HRR22_RS04125	888443..889087	+	645	WP_039025881.1	hypothetical protein	-
HRR22_RS04130	889137..889505	+	369	WP_039025882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRR22_RS04135	889594..889971	+	378	WP_039025883.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HRR22_RS04140	889968..890456	+	489	Protein_801	hypothetical protein	-
HRR22_RS04145	890476..890673	+	198	WP_000772024.1	DUF957 domain-containing protein	-
HRR22_RS04150	890770..891618	+	849	WP_039025884.1	DUF4942 domain-containing protein	-
HRR22_RS04155	891711..893030	-	1320	WP_000144688.1	site-specific integrase	-
HRR22_RS04160	893368..893706	-	339	Protein_805	LysR family transcriptional regulator	-
HRR22_RS04165	893757..894713	-	957	WP_000121330.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	887162..923806	36644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14176.22 Da
• Isoelectric Point: 7.8523

>T33522 WP_039025883.1 NZ_AP021890:889594-889971 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T33522 NZ_AP021890:889594-889971 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13792.52 Da
• Isoelectric Point: 5.9582

>AT33522 WP_039025882.1 NZ_AP021890:889137-889505 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT33522 NZ_AP021890:889137-889505 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A2X7CAN1

Antitoxin

• AlphaFold DB: A0A2X5LBZ2