Detailed information of TA system TA033501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 16122..16603
• Replicon: plasmid SGTM_pl2
• Accession: NZ_AP021883
• Organism: Sulfuriferula nivalis strain SGTM

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: SFSGTM_RS16935
• Protein ID: WP_162084759.1
• Coordinates: 16122..16403 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: SFSGTM_RS16940
• Protein ID: WP_162084760.1
• Coordinates: 16403..16603 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFSGTM_RS16900	11245..11445	+	201	WP_162086453.1	chromosome partitioning protein ParB	-
SFSGTM_RS16905	11548..11940	+	393	WP_162086454.1	hypothetical protein	-
SFSGTM_RS16910	11948..12181	-	234	WP_162086455.1	hypothetical protein	-
SFSGTM_RS16915	12269..12619	-	351	WP_162086456.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SFSGTM_RS16920	12586..13020	-	435	WP_162086463.1	hypothetical protein	-
SFSGTM_RS16925	13385..13939	-	555	WP_162086457.1	replication/maintenance protein RepL	-
SFSGTM_RS16930	15520..16077	-	558	WP_162084758.1	recombinase family protein	-
SFSGTM_RS16935	16122..16403	-	282	WP_162084759.1	virulence factor	Toxin
SFSGTM_RS16940	16403..16603	-	201	WP_162084760.1	DUF5397 domain-containing protein	Antitoxin
SFSGTM_RS16945	16762..19740	+	2979	WP_162084761.1	Tn3 family transposase	-
SFSGTM_RS16950	19773..19934	+	162	Protein_22	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28585	28585
	-	inside	IScluster/Tn	-	-	15520..19740	4220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

• Protein of unknown function DUF5397 (6-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10556.00 Da
• Isoelectric Point: 6.4608

>T33501 WP_162084759.1 NZ_AP021883:c16403-16122 [Sulfuriferula nivalis]
MYAVSFDLTVADAEMNHPKGVSQAYTEIGAILGEHGFRRVQGSLYVTDNEDMANLFLAFQTLRARPWFPKSVRDIRAFRI
EQWSDFTAVVKGV

Nucleotide

• Length: 282 bp

>T33501 NZ_AP021883:c16403-16122 [Sulfuriferula nivalis]
ATGTACGCAGTTTCCTTTGATCTGACGGTGGCCGACGCCGAGATGAACCACCCAAAAGGGGTTTCACAGGCATACACCGA
GATCGGTGCCATCTTGGGTGAACATGGTTTTCGCCGAGTTCAAGGTAGCTTGTATGTGACAGACAACGAGGACATGGCCA
ATCTCTTCCTTGCCTTCCAAACGTTGCGTGCTCGTCCTTGGTTTCCGAAGTCCGTACGAGATATCCGAGCCTTCCGCATT
GAGCAATGGTCAGATTTTACCGCCGTGGTTAAAGGAGTCTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7355.34 Da
• Isoelectric Point: 4.6855

>AT33501 WP_162084760.1 NZ_AP021883:c16603-16403 [Sulfuriferula nivalis]
MQAVTTAPPIPTGKIKSFGAFGPKYEVGKPLRQLDDGDWMVEVKMVETGESAEYRLTHVYDDPEAR

Nucleotide

• Length: 201 bp

>AT33501 NZ_AP021883:c16603-16403 [Sulfuriferula nivalis]
ATGCAAGCCGTGACGACTGCCCCGCCCATACCCACGGGCAAGATCAAGAGCTTTGGTGCGTTCGGGCCAAAGTACGAAGT
TGGCAAGCCACTTCGCCAGTTGGATGATGGTGACTGGATGGTCGAGGTCAAGATGGTCGAGACAGGCGAGTCCGCCGAAT
ATCGGTTGACACATGTTTACGATGATCCAGAGGCTCGCTAA