Detailed information of TA system TA033500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 39193..39674
• Replicon: plasmid SGTM_pl1
• Accession: NZ_AP021882
• Organism: Sulfuriferula nivalis strain SGTM

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: SFSGTM_RS16785
• Protein ID: WP_162084759.1
• Coordinates: 39393..39674 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: SFSGTM_RS16780
• Protein ID: WP_162084760.1
• Coordinates: 39193..39393 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFSGTM_RS16765	34714..35358	+	645	WP_162086426.1	hypothetical protein	-
SFSGTM_RS16770	35475..35990	-	516	WP_162086427.1	hypothetical protein	-
SFSGTM_RS16775	36056..39034	-	2979	WP_162084761.1	Tn3 family transposase	-
SFSGTM_RS16780	39193..39393	+	201	WP_162084760.1	DUF5397 domain-containing protein	Antitoxin
SFSGTM_RS16785	39393..39674	+	282	WP_162084759.1	virulence factor	Toxin
SFSGTM_RS16790	39719..40276	+	558	WP_162084758.1	recombinase family protein	-
SFSGTM_RS16795	40283..40615	-	333	WP_162086428.1	hypothetical protein	-
SFSGTM_RS16800	40755..41285	-	531	WP_162086429.1	hypothetical protein	-
SFSGTM_RS16805	41552..41962	+	411	WP_162086430.1	hypothetical protein	-
SFSGTM_RS16810	42092..43027	+	936	WP_179954410.1	DnaJ domain-containing protein	-
SFSGTM_RS16815	43112..43474	+	363	WP_162086431.1	hypothetical protein	-
SFSGTM_RS16820	43563..43964	-	402	WP_162086432.1	type II toxin-antitoxin system MqsA family antitoxin	-
SFSGTM_RS16825	43967..44269	-	303	WP_162086433.1	type II toxin-antitoxin system MqsR family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45416	45416
	-	inside	IScluster/Tn	-	-	36056..40276	4220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

• Protein of unknown function DUF5397 (6-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10556.00 Da
• Isoelectric Point: 6.4608

>T33500 WP_162084759.1 NZ_AP021882:39393-39674 [Sulfuriferula nivalis]
MYAVSFDLTVADAEMNHPKGVSQAYTEIGAILGEHGFRRVQGSLYVTDNEDMANLFLAFQTLRARPWFPKSVRDIRAFRI
EQWSDFTAVVKGV

Nucleotide

• Length: 282 bp

>T33500 NZ_AP021882:39393-39674 [Sulfuriferula nivalis]
ATGTACGCAGTTTCCTTTGATCTGACGGTGGCCGACGCCGAGATGAACCACCCAAAAGGGGTTTCACAGGCATACACCGA
GATCGGTGCCATCTTGGGTGAACATGGTTTTCGCCGAGTTCAAGGTAGCTTGTATGTGACAGACAACGAGGACATGGCCA
ATCTCTTCCTTGCCTTCCAAACGTTGCGTGCTCGTCCTTGGTTTCCGAAGTCCGTACGAGATATCCGAGCCTTCCGCATT
GAGCAATGGTCAGATTTTACCGCCGTGGTTAAAGGAGTCTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7355.34 Da
• Isoelectric Point: 4.6855

>AT33500 WP_162084760.1 NZ_AP021882:39193-39393 [Sulfuriferula nivalis]
MQAVTTAPPIPTGKIKSFGAFGPKYEVGKPLRQLDDGDWMVEVKMVETGESAEYRLTHVYDDPEAR

Nucleotide

• Length: 201 bp

>AT33500 NZ_AP021882:39193-39393 [Sulfuriferula nivalis]
ATGCAAGCCGTGACGACTGCCCCGCCCATACCCACGGGCAAGATCAAGAGCTTTGGTGCGTTCGGGCCAAAGTACGAAGT
TGGCAAGCCACTTCGCCAGTTGGATGATGGTGACTGGATGGTCGAGGTCAAGATGGTCGAGACAGGCGAGTCCGCCGAAT
ATCGGTTGACACATGTTTACGATGATCCAGAGGCTCGCTAA