Detailed information of TA system TA033499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 33659..34261
• Replicon: plasmid SGTM_pl1
• Accession: NZ_AP021882
• Organism: Sulfuriferula nivalis strain SGTM

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SFSGTM_RS16760
• Protein ID: WP_162086425.1
• Coordinates: 33965..34261 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: SFSGTM_RS16755
• Protein ID: WP_162086424.1
• Coordinates: 33659..33961 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFSGTM_RS16740	30094..30960	+	867	WP_162086422.1	DUF4123 domain-containing protein	-
SFSGTM_RS16745	30950..31486	+	537	WP_162086423.1	DUF3304 domain-containing protein	-
SFSGTM_RS16750	31585..33147	+	1563	WP_162086439.1	DUF2235 domain-containing protein	-
SFSGTM_RS16755	33659..33961	-	303	WP_162086424.1	putative addiction module antidote protein	Antitoxin
SFSGTM_RS16760	33965..34261	-	297	WP_162086425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SFSGTM_RS16765	34714..35358	+	645	WP_162086426.1	hypothetical protein	-
SFSGTM_RS16770	35475..35990	-	516	WP_162086427.1	hypothetical protein	-
SFSGTM_RS16775	36056..39034	-	2979	WP_162084761.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45416	45416
	-	inside	IScluster/Tn	-	-	36056..40276	4220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11035.48 Da
• Isoelectric Point: 4.8484

>T33499 WP_162086425.1 NZ_AP021882:c34261-33965 [Sulfuriferula nivalis]
MNSIHTTEIFDDWFASLKDKQAVRRVQVRIDRAEDGNFGDCKPVGKGVSEMRIDYGPGYRVYFVQQGIEIVILLAGGDKS
TQSKDIKTALEIAQQIEE

Nucleotide

• Length: 297 bp

>T33499 NZ_AP021882:c34261-33965 [Sulfuriferula nivalis]
ATGAATTCAATTCACACAACGGAAATTTTCGATGACTGGTTCGCTAGTCTGAAGGATAAGCAGGCCGTGAGGCGTGTACA
GGTTCGAATCGATCGCGCCGAAGATGGTAATTTTGGGGATTGCAAACCTGTTGGCAAAGGCGTTTCCGAAATGCGGATTG
ATTATGGACCAGGCTATCGCGTGTACTTTGTTCAACAAGGAATTGAAATAGTGATCTTACTGGCTGGTGGAGATAAGTCC
ACGCAATCGAAAGACATTAAAACCGCGTTGGAGATTGCGCAACAAATTGAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10817.48 Da
• Isoelectric Point: 7.2770

>AT33499 WP_162086424.1 NZ_AP021882:c33961-33659 [Sulfuriferula nivalis]
MKTIKLRKWDSAEHLKTDEDMVLYFEACLDEAGNDAAFITKALGTIARAKGMTQLAKDSGLGRESLYKALSGEGNPSFST
ILKVMSALGIKLHAEMAHSA

Nucleotide

• Length: 303 bp

>AT33499 NZ_AP021882:c33961-33659 [Sulfuriferula nivalis]
ATGAAAACGATCAAACTACGAAAATGGGATAGTGCTGAACACCTCAAGACTGATGAGGATATGGTGCTCTATTTTGAAGC
ATGTCTAGACGAGGCGGGTAATGATGCGGCTTTTATTACCAAAGCACTCGGTACGATTGCACGCGCCAAAGGCATGACAC
AACTTGCCAAAGATAGTGGATTGGGACGAGAAAGCCTCTACAAGGCCTTATCTGGCGAAGGAAATCCAAGCTTCTCTACT
ATTCTCAAGGTGATGTCAGCACTGGGTATCAAACTGCACGCAGAGATGGCTCATTCCGCATAA