Detailed information of TA system TA033495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 3149724..3150205
• Replicon: chromosome
• Accession: NZ_AP021881
• Organism: Sulfuriferula nivalis strain SGTM

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: SFSGTM_RS15545
• Protein ID: WP_162084759.1
• Coordinates: 3149924..3150205 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: SFSGTM_RS15540
• Protein ID: WP_162084760.1
• Coordinates: 3149724..3149924 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFSGTM_RS15520	3144993..3145352	-	360	WP_162083704.1	IS66 family insertion sequence element accessory protein TnpB	-
SFSGTM_RS15525	3145349..3145654	-	306	WP_162083703.1	IS66 family insertion sequence element accessory protein TnpB	-
SFSGTM_RS15530	3145681..3146418	-	738	WP_162085948.1	hypothetical protein	-
SFSGTM_RS15535	3146587..3149565	-	2979	WP_162084761.1	Tn3 family transposase	-
SFSGTM_RS15540	3149724..3149924	+	201	WP_162084760.1	DUF5397 domain-containing protein	Antitoxin
SFSGTM_RS15545	3149924..3150205	+	282	WP_162084759.1	virulence factor	Toxin
SFSGTM_RS15550	3150250..3150807	+	558	WP_162084758.1	recombinase family protein	-
SFSGTM_RS15555	3151337..3152215	+	879	WP_162085949.1	hypothetical protein	-
SFSGTM_RS15560	3152319..3153248	+	930	WP_162085950.1	LysR family transcriptional regulator	-
SFSGTM_RS15565	3153600..3154523	-	924	WP_162085951.1	class II fructose-bisphosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3146587..3150807	4220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

• Protein of unknown function DUF5397 (6-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10556.00 Da
• Isoelectric Point: 6.4608

>T33495 WP_162084759.1 NZ_AP021881:3149924-3150205 [Sulfuriferula nivalis]
MYAVSFDLTVADAEMNHPKGVSQAYTEIGAILGEHGFRRVQGSLYVTDNEDMANLFLAFQTLRARPWFPKSVRDIRAFRI
EQWSDFTAVVKGV

Nucleotide

• Length: 282 bp

>T33495 NZ_AP021881:3149924-3150205 [Sulfuriferula nivalis]
ATGTACGCAGTTTCCTTTGATCTGACGGTGGCCGACGCCGAGATGAACCACCCAAAAGGGGTTTCACAGGCATACACCGA
GATCGGTGCCATCTTGGGTGAACATGGTTTTCGCCGAGTTCAAGGTAGCTTGTATGTGACAGACAACGAGGACATGGCCA
ATCTCTTCCTTGCCTTCCAAACGTTGCGTGCTCGTCCTTGGTTTCCGAAGTCCGTACGAGATATCCGAGCCTTCCGCATT
GAGCAATGGTCAGATTTTACCGCCGTGGTTAAAGGAGTCTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7355.34 Da
• Isoelectric Point: 4.6855

>AT33495 WP_162084760.1 NZ_AP021881:3149724-3149924 [Sulfuriferula nivalis]
MQAVTTAPPIPTGKIKSFGAFGPKYEVGKPLRQLDDGDWMVEVKMVETGESAEYRLTHVYDDPEAR

Nucleotide

• Length: 201 bp

>AT33495 NZ_AP021881:3149724-3149924 [Sulfuriferula nivalis]
ATGCAAGCCGTGACGACTGCCCCGCCCATACCCACGGGCAAGATCAAGAGCTTTGGTGCGTTCGGGCCAAAGTACGAAGT
TGGCAAGCCACTTCGCCAGTTGGATGATGGTGACTGGATGGTCGAGGTCAAGATGGTCGAGACAGGCGAGTCCGCCGAAT
ATCGGTTGACACATGTTTACGATGATCCAGAGGCTCGCTAA