Detailed information of TA system TA033462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 7261116..7261742
• Replicon: chromosome
• Accession: NZ_AP021879
• Organism: Desulfosarcina ovata subsp. ovata strain oXyS1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GN112_RS31805
• Protein ID: WP_155313817.1
• Coordinates: 7261116..7261406 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GN112_RS31810
• Protein ID: WP_155313818.1
• Coordinates: 7261416..7261742 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN112_RS31775	7256816..7257085	+	270	WP_155313813.1	hypothetical protein	-
GN112_RS31785	7258494..7258751	+	258	WP_155313814.1	hypothetical protein	-
GN112_RS31790	7258916..7259401	+	486	WP_162459214.1	hypothetical protein	-
GN112_RS31795	7259554..7260363	+	810	WP_155313816.1	hypothetical protein	-
GN112_RS31800	7260602..7260913	+	312	WP_197743445.1	potassium channel family protein	-
GN112_RS31805	7261116..7261406	+	291	WP_155313817.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN112_RS31810	7261416..7261742	+	327	WP_155313818.1	putative addiction module antidote protein	Antitoxin
GN112_RS31815	7262681..7263067	+	387	WP_155313819.1	hypothetical protein	-
GN112_RS31820	7263169..7264059	+	891	WP_155313820.1	DUF2971 domain-containing protein	-
GN112_RS31825	7264209..7264916	+	708	WP_155313821.1	hypothetical protein	-
GN112_RS31830	7265076..7265957	+	882	WP_155308513.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	7254543..7265957	11414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10842.69 Da
• Isoelectric Point: 10.7928

>T33462 WP_155313817.1 NZ_AP021879:7261116-7261406 [Desulfosarcina ovata subsp. ovata]
MIEIRKTETFAKWLDGLNDIRARARILVRIERLAAGNPGDVKPVGEGVSELRIDYGPGYRVYYKKRGSKLIILLAGGDKR
TQSKDIKFALRLAQNL

Nucleotide

• Length: 291 bp

>T33462 NZ_AP021879:7261116-7261406 [Desulfosarcina ovata subsp. ovata]
ATGATCGAAATCCGTAAGACTGAGACTTTTGCCAAGTGGCTTGACGGTTTGAACGATATCCGTGCACGTGCTCGTATTTT
GGTAAGGATCGAACGGTTGGCAGCAGGTAATCCTGGTGATGTTAAACCTGTCGGCGAAGGAGTTTCAGAGTTACGTATCG
ACTACGGCCCAGGTTACCGCGTTTATTACAAAAAGCGAGGCAGCAAATTGATCATTCTATTGGCTGGTGGCGATAAGCGC
ACTCAATCCAAGGACATAAAATTCGCATTGCGCCTTGCTCAGAATCTATAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11662.34 Da
• Isoelectric Point: 6.7186

>AT33462 WP_155313818.1 NZ_AP021879:7261416-7261742 [Desulfosarcina ovata subsp. ovata]
MSKIITTRYDVAEHLRTPEEMAAYLEACFEEASGDAAFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERRPTFDT
ILKVIGALGLKLHAEAIHTNINTGQPVT

Nucleotide

• Length: 327 bp

>AT33462 NZ_AP021879:7261416-7261742 [Desulfosarcina ovata subsp. ovata]
ATGTCTAAAATCATCACAACACGCTACGATGTCGCTGAGCATCTTCGGACGCCGGAGGAAATGGCAGCCTATCTTGAAGC
TTGTTTCGAGGAGGCATCAGGTGATGCTGCTTTCATTGCCAAAGCCCTGGGTGATATCGCACGCGCAAAAGGCATGTCGC
AGGTGGCTCGTGATGCCGGACTTTCCAGAGAAAGTCTTTATAAAGCCCTGTCAGGAGAACGAAGGCCAACCTTCGATACA
ATTCTTAAGGTGATCGGAGCACTTGGCCTAAAATTGCATGCGGAAGCGATTCATACAAATATCAATACGGGCCAACCTGT
CACTTAA