Detailed information of TA system TA033459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 5979661..5980245
• Replicon: chromosome
• Accession: NZ_AP021879
• Organism: Desulfosarcina ovata subsp. ovata strain oXyS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GN112_RS26265
• Protein ID: WP_155312876.1
• Coordinates: 5979661..5979996 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GN112_RS26270
• Protein ID: WP_155312877.1
• Coordinates: 5979997..5980245 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN112_RS26230	5974822..5975052	-	231	WP_155309029.1	hypothetical protein	-
GN112_RS26235	5975282..5975647	+	366	WP_197743251.1	hypothetical protein	-
GN112_RS26240	5975652..5976227	+	576	WP_155309688.1	DNA methylase	-
GN112_RS26245	5976208..5977887	+	1680	WP_155309035.1	integrase	-
GN112_RS26250	5977863..5978531	+	669	WP_155309036.1	ATP-binding protein	-
GN112_RS26255	5978969..5979226	-	258	WP_155312874.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GN112_RS26260	5979213..5979446	-	234	WP_155312875.1	CopG family transcriptional regulator	-
GN112_RS26265	5979661..5979996	-	336	WP_155312876.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GN112_RS26270	5979997..5980245	-	249	WP_155312877.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GN112_RS26275	5980382..5980702	-	321	WP_155312878.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GN112_RS26280	5980699..5980929	-	231	WP_155312879.1	addiction module protein	-
GN112_RS26285	5982166..5982561	-	396	WP_155312880.1	ATP-binding protein	-
GN112_RS26290	5982719..5983981	+	1263	WP_155312881.1	tyrosine-type recombinase/integrase	-
GN112_RS26295	5983968..5984942	+	975	WP_155312882.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5950862..6001971	51109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-107)

Antitoxin

• Antitoxin PemI-like (1-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12423.36 Da
• Isoelectric Point: 9.1717

>T33459 WP_155312876.1 NZ_AP021879:c5979996-5979661 [Desulfosarcina ovata subsp. ovata]
MAAYVPKQGDIIAITFDPQSGHEQKGRRPAFVVSKDLFNRSTGLAIVCPVTNTERGFPFHVPIPKDSKLTGFIMVEQVKS
VDFRARRAKRIERSSDELLSEVLSILDACIY

Nucleotide

• Length: 336 bp

>T33459 NZ_AP021879:c5979996-5979661 [Desulfosarcina ovata subsp. ovata]
ATGGCTGCTTATGTCCCCAAGCAAGGGGACATTATCGCAATCACATTTGATCCACAATCTGGTCATGAGCAGAAGGGGCG
ACGCCCTGCTTTTGTTGTCAGCAAGGACCTATTCAATCGAAGCACAGGTTTGGCTATTGTGTGCCCGGTAACAAACACAG
AGCGAGGTTTTCCCTTCCACGTTCCTATACCAAAAGACAGCAAGCTGACCGGTTTCATTATGGTTGAGCAAGTTAAATCT
GTCGATTTTCGAGCTCGTCGTGCCAAACGAATTGAACGTAGCAGTGATGAACTCCTATCTGAAGTTCTGTCCATACTGGA
TGCATGCATTTATTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9086.53 Da
• Isoelectric Point: 9.6909

>AT33459 WP_155312877.1 NZ_AP021879:c5980245-5979997 [Desulfosarcina ovata subsp. ovata]
MLAKIQKWGNSQGLRLTKNLLADAQLGVGDEVDISIKDGIMIVTPAKKIRGKHSLKDLVARIPENYKTGEVDWSDPVGKE
TW

Nucleotide

• Length: 249 bp

>AT33459 NZ_AP021879:c5980245-5979997 [Desulfosarcina ovata subsp. ovata]
ATGCTTGCAAAAATTCAGAAATGGGGAAACAGCCAAGGGCTTCGTCTTACAAAAAACCTGCTCGCCGATGCGCAGCTTGG
AGTCGGTGACGAGGTGGATATCAGTATAAAAGATGGTATTATGATTGTCACGCCGGCCAAAAAGATACGCGGCAAACACA
GTCTCAAGGATCTTGTCGCACGTATTCCTGAAAATTACAAAACCGGCGAAGTCGACTGGAGTGATCCGGTTGGCAAGGAG
ACTTGGTAA