Detailed information of TA system TA033444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 919366..920008
• Replicon: chromosome
• Accession: NZ_AP021879
• Organism: Desulfosarcina ovata subsp. ovata strain oXyS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GN112_RS04025
• Protein ID: WP_162458775.1
• Coordinates: 919366..919758 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GN112_RS04030
• Protein ID: WP_155309047.1
• Coordinates: 919778..920008 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN112_RS04000	914995..916557	+	1563	WP_162458774.1	DDE-type integrase/transposase/recombinase	-
GN112_RS04005	916554..917288	+	735	WP_155309044.1	AAA family ATPase	-
GN112_RS04010	917302..917760	+	459	WP_155309045.1	hypothetical protein	-
GN112_RS04020	918690..919338	+	649	Protein_796	radical SAM protein	-
GN112_RS04025	919366..919758	-	393	WP_162458775.1	type II toxin-antitoxin system VapC family toxin	Toxin
GN112_RS04030	919778..920008	-	231	WP_155309047.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GN112_RS04035	920339..920989	+	651	WP_155309048.1	helix-turn-helix domain-containing protein	-
GN112_RS04040	920994..921248	+	255	WP_155309049.1	hypothetical protein	-
GN112_RS04045	921766..921957	+	192	WP_155309050.1	ferredoxin	-
GN112_RS04050	922004..922579	+	576	WP_155309051.1	rubrerythrin family protein	-
GN112_RS04055	922740..924806	-	2067	WP_155309052.1	elongation factor G	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	889400..922579	33179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14926.10 Da
• Isoelectric Point: 8.8266

>T33444 WP_162458775.1 NZ_AP021879:c919758-919366 [Desulfosarcina ovata subsp. ovata]
MDTNIVSYWMRGDSAIIQKIQTFSPSDLSLSTVTLAEIYYGIEKSPTRKKERQLKIERIKSQLEIYPFNESAALKYGSIR
ASLEKQGQPISERDLQIAAIALANKFCVVTHNVKEFRRITDLKVEDWAQD

Nucleotide

• Length: 393 bp

>T33444 NZ_AP021879:c919758-919366 [Desulfosarcina ovata subsp. ovata]
TTGGATACCAATATCGTCAGCTATTGGATGCGTGGTGACTCCGCCATCATTCAGAAAATACAAACCTTCAGTCCATCCGA
TCTATCGCTCTCGACGGTTACGCTGGCCGAAATCTATTATGGTATCGAAAAATCGCCCACCAGGAAAAAAGAACGACAGC
TGAAAATCGAGCGCATAAAGTCCCAGTTGGAGATATATCCGTTCAATGAATCGGCTGCGCTTAAATACGGCAGTATCCGG
GCATCACTCGAAAAGCAGGGGCAGCCGATCAGTGAAAGAGATCTGCAAATTGCCGCCATCGCGCTTGCCAACAAGTTTTG
TGTTGTAACGCACAACGTTAAAGAATTTCGCCGAATTACCGATTTAAAGGTCGAAGACTGGGCGCAGGATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8791.19 Da
• Isoelectric Point: 9.9496

>AT33444 WP_155309047.1 NZ_AP021879:c920008-919778 [Desulfosarcina ovata subsp. ovata]
MESVAKIFMNGRSQAVRLPKAFRFEGSEVRIRKQGEKVILEPIKKTKWPDGFWDIFTPDPQFETPAPLPPKDFSLD

Nucleotide

• Length: 231 bp

>AT33444 NZ_AP021879:c920008-919778 [Desulfosarcina ovata subsp. ovata]
ATGGAATCTGTAGCCAAGATTTTCATGAATGGAAGATCCCAGGCGGTTCGGCTGCCAAAAGCGTTTCGATTCGAAGGCTC
TGAGGTCAGAATCCGAAAACAGGGGGAGAAAGTCATTCTGGAGCCAATTAAAAAAACAAAGTGGCCGGATGGATTCTGGG
ACATTTTTACGCCAGACCCCCAGTTTGAAACGCCGGCCCCATTGCCCCCCAAAGACTTCTCCCTGGATTAA