Detailed information of TA system TA033439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 27518..27952
• Replicon: plasmid Do28_1
• Accession: NZ_AP021877
• Organism: Desulfosarcina ovata subsp. sediminis strain 28bB2T

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GN113_RS36040
• Protein ID: WP_155326274.1
• Coordinates: 27728..27952 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GN113_RS36035
• Protein ID: WP_155326273.1
• Coordinates: 27518..27727 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN113_RS36025	23570..24523	+	954	WP_155326271.1	tyrosine-type recombinase/integrase	-
GN113_RS36030	24501..27506	+	3006	WP_155326272.1	Tn3 family transposase	-
GN113_RS36035	27518..27727	+	210	WP_155326273.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GN113_RS36040	27728..27952	+	225	WP_155326274.1	type II toxin-antitoxin system HicA family toxin	Toxin
GN113_RS36045	28150..28536	-	387	WP_155326275.1	VOC family protein	-
GN113_RS36050	28540..28875	-	336	WP_155326276.1	cupin domain-containing protein	-
GN113_RS36055	29006..29566	-	561	WP_155326277.1	hemerythrin domain-containing protein	-
GN113_RS36060	29621..31918	-	2298	WP_155326278.1	nitric-oxide reductase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135961	135961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-65)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8492.95 Da
• Isoelectric Point: 10.9142

>T33439 WP_155326274.1 NZ_AP021877:27728-27952 [Desulfosarcina ovata subsp. sediminis]
MGNVPILKPKEIIAILTKLGFQEIRQRGSHKQFRHVDGRGTTVPFHSSRDISPILLRQICKDIGLTIEEFLQNR

Nucleotide

• Length: 225 bp

>T33439 NZ_AP021877:27728-27952 [Desulfosarcina ovata subsp. sediminis]
ATGGGGAATGTTCCGATTCTCAAGCCAAAAGAGATTATCGCAATTTTAACGAAACTTGGCTTCCAGGAAATTCGTCAGCG
TGGATCGCACAAGCAATTTAGGCATGTCGACGGCCGAGGAACCACGGTTCCGTTTCATTCGTCAAGGGATATTTCTCCGA
TCTTGCTGCGTCAAATCTGCAAGGACATTGGGCTCACAATAGAAGAGTTTTTGCAAAACCGATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7624.78 Da
• Isoelectric Point: 4.0857

>AT33439 WP_155326273.1 NZ_AP021877:27518-27727 [Desulfosarcina ovata subsp. sediminis]
MKIFTAVVEKCPETNLFVGYVPGFPGAHTQAETLDELNKNLKEVIEMLLEDGDPKLEAEFIGTQNVMVA

Nucleotide

• Length: 210 bp

>AT33439 NZ_AP021877:27518-27727 [Desulfosarcina ovata subsp. sediminis]
ATGAAAATCTTTACGGCGGTCGTGGAAAAATGCCCGGAAACAAACCTTTTTGTCGGTTATGTCCCAGGCTTTCCTGGTGC
CCATACTCAAGCGGAAACCTTGGACGAGCTTAATAAGAATCTCAAGGAGGTCATTGAGATGCTTCTTGAAGATGGCGATC
CAAAGCTTGAGGCGGAATTTATCGGCACCCAAAATGTCATGGTGGCCTAA