Detailed information of TA system TA033433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 5129912..5130600
• Replicon: chromosome
• Accession: NZ_AP021876
• Organism: Desulfosarcina ovata subsp. sediminis strain 28bB2T

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GN113_RS22330
• Protein ID: WP_155324020.1
• Coordinates: 5130160..5130600 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GN113_RS22325
• Protein ID: WP_155324019.1
• Coordinates: 5129912..5130163 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN113_RS22295	5125658..5126029	-	372	WP_155320799.1	transposase zinc-binding domain-containing protein	-
GN113_RS22300	5126082..5126267	+	186	WP_155324015.1	hypothetical protein	-
GN113_RS22305	5126422..5126772	+	351	WP_155324016.1	hypothetical protein	-
GN113_RS22310	5126846..5128186	-	1341	WP_155321404.1	group II intron reverse transcriptase/maturase	-
GN113_RS22315	5129004..5129282	+	279	WP_155324017.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GN113_RS22320	5129282..5129572	+	291	WP_155324018.1	HigA family addiction module antidote protein	-
GN113_RS22325	5129912..5130163	+	252	WP_155324019.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GN113_RS22330	5130160..5130600	+	441	WP_155324020.1	type II toxin-antitoxin system VapC family toxin	Toxin
GN113_RS22335	5130674..5132014	-	1341	WP_173179719.1	group II intron reverse transcriptase/maturase	-
GN113_RS22340	5132242..5133284	+	1043	Protein_4451	IS630 family transposase	-
GN113_RS22345	5133841..5134032	-	192	WP_155324021.1	hypothetical protein	-
GN113_RS22350	5134486..5134815	+	330	WP_173179721.1	DUF4258 domain-containing protein	-
GN113_RS22355	5134812..5135027	+	216	WP_020587764.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-68)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16467.73 Da
• Isoelectric Point: 7.2764

>T33433 WP_155324020.1 NZ_AP021876:5130160-5130600 [Desulfosarcina ovata subsp. sediminis]
MNYLLDTCVISELVKTTPNGNVINWINHTPNERLFLSFITIGEIRKGITKLTDSKKKHRLTNWLNTLLEDYEARIYPVDL
AVAESWGSIQGKAENNGTPVASVDSLIAAVAQTHNLIVVTRNEKDFAATNVTILNPWKDKGYRDRE

Nucleotide

• Length: 441 bp

>T33433 NZ_AP021876:5130160-5130600 [Desulfosarcina ovata subsp. sediminis]
TTGAATTATTTATTAGATACTTGTGTGATATCAGAATTGGTCAAGACAACTCCAAATGGAAACGTCATAAATTGGATAAA
TCATACACCGAATGAAAGACTTTTTTTATCTTTCATAACCATCGGAGAAATACGTAAAGGTATTACCAAACTTACTGACT
CAAAAAAGAAACATCGGCTTACAAACTGGCTTAATACATTATTGGAAGATTACGAAGCAAGAATCTACCCCGTTGATTTG
GCCGTTGCAGAAAGTTGGGGGAGTATTCAGGGAAAAGCAGAGAACAACGGTACTCCTGTCGCATCCGTTGATAGTTTGAT
AGCAGCAGTGGCTCAAACACACAATTTAATTGTCGTAACAAGAAATGAAAAAGACTTTGCCGCAACAAATGTTACAATCT
TGAATCCATGGAAAGATAAAGGATATCGGGATAGGGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9768.26 Da
• Isoelectric Point: 9.4876

>AT33433 WP_155324019.1 NZ_AP021876:5129912-5130163 [Desulfosarcina ovata subsp. sediminis]
MNTSWKLQDAKAKFSQVVENALKMGPQYVTRRGQDAVVILSVKEYQKITTKKPTFKEFLLSCPKMDDGFEFERQKDYPRD
IEF

Nucleotide

• Length: 252 bp

>AT33433 NZ_AP021876:5129912-5130163 [Desulfosarcina ovata subsp. sediminis]
ATGAATACATCATGGAAATTACAAGATGCTAAGGCAAAATTCAGTCAGGTAGTCGAAAATGCTTTGAAAATGGGACCACA
ATATGTAACACGTAGAGGCCAGGATGCTGTAGTCATCTTATCCGTTAAAGAATATCAAAAAATCACCACAAAAAAACCGA
CATTTAAAGAGTTTCTTTTAAGTTGTCCCAAAATGGATGATGGTTTTGAATTTGAAAGACAAAAAGATTATCCGAGGGAT
ATTGAATTTTGA