Detailed information of TA system TA033427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3013021..3013667
• Replicon: chromosome
• Accession: NZ_AP021876
• Organism: Desulfosarcina ovata subsp. sediminis strain 28bB2T

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GN113_RS13420
• Protein ID: WP_197910464.1
• Coordinates: 3013021..3013332 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GN113_RS13425
• Protein ID: WP_173179393.1
• Coordinates: 3013329..3013667 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN113_RS13405	3008750..3009703	-	954	WP_155322669.1	type I-B CRISPR-associated protein Cas7/Csh2	-
GN113_RS13410	3009723..3011720	-	1998	WP_173179391.1	CRISPR-associated protein	-
GN113_RS13415	3011902..3012564	-	663	WP_155322671.1	DNA repair protein	-
GN113_RS13420	3013021..3013332	+	312	WP_197910464.1	type II toxin-antitoxin system HicA family toxin	Toxin
GN113_RS13425	3013329..3013667	+	339	WP_173179393.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GN113_RS13430	3013664..3013963	-	300	WP_155310550.1	CRISPR-associated endonuclease Cas2	-
GN113_RS13435	3013966..3014982	-	1017	WP_155322672.1	CRISPR-associated endonuclease Cas1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (42-94)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11735.52 Da
• Isoelectric Point: 10.6053

>T33427 WP_197910464.1 NZ_AP021876:3013021-3013332 [Desulfosarcina ovata subsp. sediminis]
VYERHLFLETVLIYGTISTVKRKHQKTLDAIFIRPVGGNIKWRDIEALFVELGAELSEREGSRIGVRLFGERRVFHRPHP
SPNTDKGAVASIRKWLADNGVTP

Nucleotide

• Length: 312 bp

>T33427 NZ_AP021876:3013021-3013332 [Desulfosarcina ovata subsp. sediminis]
GTGTATGAAAGGCATTTATTTCTTGAAACGGTACTGATATATGGTACTATTTCTACTGTGAAACGCAAACACCAAAAAAC
CTTGGACGCCATATTTATAAGACCGGTGGGAGGTAATATTAAATGGCGTGATATTGAAGCCTTGTTCGTTGAATTGGGTG
CCGAATTAAGCGAACGTGAAGGTTCACGAATCGGTGTTCGCCTGTTTGGTGAAAGGAGAGTGTTTCACCGACCGCATCCT
TCGCCGAATACGGACAAAGGGGCTGTCGCCAGCATTCGAAAATGGTTGGCGGACAACGGAGTAACACCATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12476.15 Da
• Isoelectric Point: 4.4606

>AT33427 WP_173179393.1 NZ_AP021876:3013329-3013667 [Desulfosarcina ovata subsp. sediminis]
MMNIMEINGYQAVIKYDPEIEMFRGEFIGLNGGADFYAGDIDGLRREGETSLKVFLDMCKEDGVDPRKAFSGKFNLRVSP
QLHARIVTRAAAEGKSLNQWVADLLDESISAD

Nucleotide

• Length: 339 bp

>AT33427 NZ_AP021876:3013329-3013667 [Desulfosarcina ovata subsp. sediminis]
ATGATGAACATTATGGAAATCAATGGCTATCAGGCGGTCATCAAATATGACCCGGAAATTGAAATGTTTCGTGGAGAATT
CATCGGTCTTAACGGCGGAGCCGATTTTTATGCCGGCGACATCGATGGGCTTCGCAGAGAGGGAGAAACGTCACTAAAGG
TATTCCTGGACATGTGTAAGGAAGACGGGGTGGATCCGCGCAAGGCGTTTTCCGGTAAATTCAACTTGCGTGTATCGCCA
CAGTTGCACGCTCGTATCGTTACTCGCGCCGCCGCAGAGGGGAAAAGCCTGAATCAGTGGGTAGCGGATCTGCTCGATGA
GTCCATCAGCGCCGATTAA