Detailed information of TA system TA033426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2308350..2308976
• Replicon: chromosome
• Accession: NZ_AP021876
• Organism: Desulfosarcina ovata subsp. sediminis strain 28bB2T

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GN113_RS10560
• Protein ID: WP_155313817.1
• Coordinates: 2308686..2308976 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GN113_RS10555
• Protein ID: WP_155313818.1
• Coordinates: 2308350..2308676 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN113_RS10550	2304925..2308137	-	3213	WP_155322231.1	hypothetical protein	-
GN113_RS10555	2308350..2308676	-	327	WP_155313818.1	putative addiction module antidote protein	Antitoxin
GN113_RS10560	2308686..2308976	-	291	WP_155313817.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN113_RS10565	2309138..2310034	-	897	WP_155322232.1	hypothetical protein	-
GN113_RS10570	2310198..2311145	-	948	WP_155322233.1	radical SAM protein	-
GN113_RS10575	2311736..2312854	-	1119	WP_155321248.1	IS110 family transposase	-
GN113_RS10580	2313276..2313740	-	465	WP_155322234.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10842.69 Da
• Isoelectric Point: 10.7928

>T33426 WP_155313817.1 NZ_AP021876:c2308976-2308686 [Desulfosarcina ovata subsp. sediminis]
MIEIRKTETFAKWLDGLNDIRARARILVRIERLAAGNPGDVKPVGEGVSELRIDYGPGYRVYYKKRGSKLIILLAGGDKR
TQSKDIKFALRLAQNL

Nucleotide

• Length: 291 bp

>T33426 NZ_AP021876:c2308976-2308686 [Desulfosarcina ovata subsp. sediminis]
ATGATCGAAATCCGTAAGACTGAGACTTTTGCCAAGTGGCTTGACGGTTTGAACGATATCCGTGCACGTGCTCGTATTTT
GGTAAGGATCGAACGGTTGGCAGCAGGTAATCCTGGTGATGTTAAACCTGTCGGCGAAGGAGTTTCAGAGTTACGTATCG
ACTACGGCCCAGGTTACCGCGTTTATTACAAAAAGCGAGGCAGCAAATTGATCATTCTATTGGCTGGTGGCGATAAGCGC
ACTCAATCCAAGGACATAAAATTCGCATTGCGCCTTGCTCAGAATCTATAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11662.34 Da
• Isoelectric Point: 6.7186

>AT33426 WP_155313818.1 NZ_AP021876:c2308676-2308350 [Desulfosarcina ovata subsp. sediminis]
MSKIITTRYDVAEHLRTPEEMAAYLEACFEEASGDAAFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERRPTFDT
ILKVIGALGLKLHAEAIHTNINTGQPVT

Nucleotide

• Length: 327 bp

>AT33426 NZ_AP021876:c2308676-2308350 [Desulfosarcina ovata subsp. sediminis]
ATGTCTAAAATCATCACAACACGCTACGATGTCGCTGAGCATCTTCGGACGCCGGAGGAAATGGCAGCCTATCTTGAAGC
TTGTTTCGAGGAGGCATCAGGTGATGCTGCTTTTATTGCCAAAGCCCTGGGTGATATCGCACGCGCAAAAGGCATGTCGC
AGGTGGCTCGTGATGCCGGACTTTCCAGAGAAAGTCTTTATAAAGCCCTGTCAGGAGAACGAAGGCCAACCTTCGATACA
ATTCTTAAGGTGATCGGAGCACTTGGCCTAAAATTGCATGCGGAAGCGATTCATACAAATATCAATACGGGCCAACCTGT
CACTTAA