Detailed information of TA system TA033422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 1551437..1551872
• Replicon: chromosome
• Accession: NZ_AP021876
• Organism: Desulfosarcina ovata subsp. sediminis strain 28bB2T

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GN113_RS07135
• Protein ID: WP_155321703.1
• Coordinates: 1551648..1551872 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GN113_RS07130
• Protein ID: WP_155321702.1
• Coordinates: 1551437..1551646 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN113_RS07120	1547473..1548426	+	954	WP_173179149.1	tyrosine-type recombinase/integrase	-
GN113_RS07125	1548404..1551406	+	3003	WP_155321701.1	Tn3 family transposase	-
GN113_RS07130	1551437..1551646	+	210	WP_155321702.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GN113_RS07135	1551648..1551872	+	225	WP_155321703.1	type II toxin-antitoxin system HicA family toxin	Toxin
GN113_RS07140	1552766..1553617	-	852	WP_155321704.1	transglutaminase family protein	-
GN113_RS07145	1553626..1555305	-	1680	WP_173179151.1	phosphoenolpyruvate--protein phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-65)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8462.92 Da
• Isoelectric Point: 10.9142

>T33422 WP_155321703.1 NZ_AP021876:1551648-1551872 [Desulfosarcina ovata subsp. sediminis]
MGNVPILKPKEVIAILTKLGFQEIRQRGSHKQFRHVDGRGTTVPFHSARDISPILLRQICKDIGLTIEEFLQNR

Nucleotide

• Length: 225 bp

>T33422 NZ_AP021876:1551648-1551872 [Desulfosarcina ovata subsp. sediminis]
ATGGGTAATGTTCCGATTCTCAAACCCAAGGAGGTTATCGCGATTTTAACGAAACTTGGCTTTCAGGAGATTCGTCAGCG
TGGATCGCATAAGCAATTTCGGCATGTCGACGGCCGAGGAACCACGGTTCCATTTCATTCGGCAAGAGATATTTCTCCGA
TCTTGCTGCGTCAAATCTGCAAGGACATTGGGCTCACAATAGAAGAGTTTTTGCAAAATCGATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7610.75 Da
• Isoelectric Point: 4.0857

>AT33422 WP_155321702.1 NZ_AP021876:1551437-1551646 [Desulfosarcina ovata subsp. sediminis]
MKVFTAVVEKCPETNLFVGYVPGFPGAHTQAETLDELNKNLKEVIEMLLEDGDPKLEAEFIGTQNVMVA

Nucleotide

• Length: 210 bp

>AT33422 NZ_AP021876:1551437-1551646 [Desulfosarcina ovata subsp. sediminis]
ATGAAAGTCTTTACAGCGGTCGTGGAAAAATGTCCGGAGACAAACCTTTTTGTCGGTTATGTTCCAGGTTTTCCTGGTGC
CCATACTCAAGCGGAAACGTTGGATGAGCTTAATAAAAATCTCAAGGAAGTAATTGAGATGCTTCTTGAAGATGGCGATC
CAAAGCTTGAGGCGGAATTTATCGGCACTCAAAATGTCATGGTGGCCTAA