Detailed information of TA system TA033421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-parD/RHH(antitoxin)
• Location: 1537796..1538321
• Replicon: chromosome
• Accession: NZ_AP021876
• Organism: Desulfosarcina ovata subsp. sediminis strain 28bB2T

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GN113_RS07080
• Protein ID: WP_197910422.1
• Coordinates: 1537796..1537990 (-)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GN113_RS07085
• Protein ID: WP_155321695.1
• Coordinates: 1538070..1538321 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN113_RS07070	1533309..1536092	-	2784	WP_155321693.1	formate dehydrogenase subunit alpha	-
GN113_RS07075	1536393..1537331	-	939	WP_155321694.1	helix-turn-helix transcriptional regulator	-
GN113_RS07080	1537796..1537990	-	195	WP_197910422.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN113_RS07085	1538070..1538321	-	252	WP_155321695.1	ribbon-helix-helix domain-containing protein	Antitoxin
GN113_RS07090	1538911..1540437	+	1527	WP_155320855.1	transposase	-
GN113_RS07095	1540683..1541027	-	345	WP_155321696.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-60)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-42)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7245.37 Da
• Isoelectric Point: 11.8138

>T33421 WP_197910422.1 NZ_AP021876:c1537990-1537796 [Desulfosarcina ovata subsp. sediminis]
VVARLWSSFNKLASHPEAGRPGRVFGARELVVSQTPFIVPYRISNSEIQILRVLHGARKWPDSF

Nucleotide

• Length: 195 bp

>T33421 NZ_AP021876:c1537990-1537796 [Desulfosarcina ovata subsp. sediminis]
GTGGTCGCCCGACTATGGTCTTCGTTCAACAAGCTTGCCAGCCATCCCGAAGCCGGTCGGCCTGGACGTGTTTTTGGAGC
CCGCGAACTAGTTGTCAGCCAGACACCGTTCATTGTTCCCTACCGGATTTCTAATTCTGAAATACAAATCCTGCGAGTGC
TGCATGGCGCAAGGAAATGGCCGGATTCATTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9318.49 Da
• Isoelectric Point: 8.5177

>AT33421 WP_155321695.1 NZ_AP021876:c1538321-1538070 [Desulfosarcina ovata subsp. sediminis]
MDTVPVSGRIDKSISEKLNLLARATARSRSFLINEAIKTYVDEQSWQVEAINEGIKQADQGRFASQAKIRKTFAKYGVNV
DED

Nucleotide

• Length: 252 bp

>AT33421 NZ_AP021876:c1538321-1538070 [Desulfosarcina ovata subsp. sediminis]
ATGGATACCGTTCCAGTCTCTGGCCGAATCGATAAATCGATATCGGAAAAATTGAACCTTCTGGCCAGGGCGACCGCCCG
CTCCCGGTCATTCCTGATCAACGAGGCCATAAAGACCTACGTGGACGAGCAATCCTGGCAAGTTGAAGCCATCAACGAAG
GTATCAAACAGGCAGATCAGGGCAGGTTTGCATCCCAGGCCAAGATCCGGAAAACATTTGCCAAATACGGCGTAAATGTT
GATGAAGATTAG