Detailed information of TA system TA033413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 386099..386739
• Replicon: chromosome
• Accession: NZ_AP021875
• Organism: Desulfosarcina widdelii strain PP31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GN111_RS01820
• Protein ID: WP_155302110.1
• Coordinates: 386326..386739 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GN111_RS01815
• Protein ID: WP_155302109.1
• Coordinates: 386099..386329 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN111_RS01780	381411..381680	+	270	WP_155302104.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GN111_RS01785	381680..381973	+	294	WP_155302105.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GN111_RS01790	382330..383667	+	1338	WP_155302106.1	MBL fold metallo-hydrolase	-
GN111_RS01795	383826..384200	+	375	WP_155302107.1	GxxExxY protein	-
GN111_RS01800	384597..384881	-	285	WP_155302108.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GN111_RS01805	384878..385147	-	270	WP_155307882.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GN111_RS01810	385205..385732	+	528	WP_197740480.1	GxxExxY protein	-
GN111_RS01815	386099..386329	+	231	WP_155302109.1	antitoxin	Antitoxin
GN111_RS01820	386326..386739	+	414	WP_155302110.1	type II toxin-antitoxin system VapC family toxin	Toxin
GN111_RS01825	387030..387326	+	297	WP_155302111.1	hypothetical protein	-
GN111_RS01830	387295..387975	+	681	WP_155302112.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
GN111_RS01835	388779..388988	+	210	WP_155302113.1	type II toxin-antitoxin system HicB family antitoxin	-
GN111_RS01840	388975..389166	+	192	WP_155302114.1	type II toxin-antitoxin system HicA family toxin	-
GN111_RS01845	389742..389996	+	255	WP_155302115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GN111_RS01850	390001..390396	+	396	WP_170302082.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	378487..442467	63980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15474.94 Da
• Isoelectric Point: 7.1349

>T33413 WP_155302110.1 NZ_AP021875:386326-386739 [Desulfosarcina widdelii]
MTVMLDTGICTYTIKRKPIGVLKRLEMCQPGTVVMSVITFSELINGAKKSQYVEDNLHRLEALSELIEVLPFDKNAAMIY
GDIRSDLENRGLIIGGNDLFIAAHALSLNYTLVTNNQREFERVRGLKTENWAVEKSR

Nucleotide

• Length: 414 bp

>T33413 NZ_AP021875:386326-386739 [Desulfosarcina widdelii]
ATGACGGTAATGCTGGATACCGGCATATGTACTTACACAATCAAGCGAAAACCGATCGGCGTTTTGAAGCGCCTTGAGAT
GTGCCAACCGGGAACGGTTGTCATGTCAGTCATCACTTTCTCGGAATTAATCAACGGGGCCAAAAAGAGCCAATACGTGG
AAGACAACCTCCACCGGTTGGAAGCGTTGAGTGAACTGATCGAGGTCCTTCCCTTCGATAAAAACGCGGCCATGATATAC
GGAGATATACGCTCGGACCTTGAAAATAGAGGGTTGATAATCGGTGGTAATGATTTATTTATCGCCGCTCATGCATTGAG
CTTGAACTATACCCTGGTGACCAACAATCAAAGGGAGTTCGAAAGGGTCAGGGGGTTGAAGACAGAAAATTGGGCTGTGG
AGAAAAGCCGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8845.15 Da
• Isoelectric Point: 7.9920

>AT33413 WP_155302109.1 NZ_AP021875:386099-386329 [Desulfosarcina widdelii]
METAKIFKNGNSQAVRLPKAFRFDVTEVQIFRRGDEVILKRKPQNLAEAFELLTRMSDDFMSEGRCQPSVQEREPL

Nucleotide

• Length: 231 bp

>AT33413 NZ_AP021875:386099-386329 [Desulfosarcina widdelii]
ATGGAAACCGCGAAAATTTTTAAAAATGGAAACTCCCAAGCGGTTCGGCTGCCCAAAGCTTTCCGCTTCGATGTCACGGA
AGTCCAGATCTTTCGCAGAGGCGATGAAGTGATTTTGAAACGGAAACCGCAGAATCTGGCAGAAGCATTCGAACTGTTGA
CCCGAATGTCGGATGATTTCATGTCAGAGGGGCGTTGCCAGCCCTCGGTACAGGAAAGAGAACCGCTATGA