Detailed information of TA system TA033408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4461224..4461819
• Replicon: chromosome
• Accession: NZ_AP021873
• Organism: Serratia marcescens strain ATCC 274

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: SMATCC274_RS21110
• Protein ID: WP_071605322.1
• Coordinates: 4461565..4461819 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: SMATCC274_RS21105
• Protein ID: WP_033633573.1
• Coordinates: 4461224..4461565 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMATCC274_RS21085	4456732..4457547	-	816	WP_161545069.1	GPO family capsid scaffolding protein	-
SMATCC274_RS21090	4457690..4459462	+	1773	WP_161545070.1	terminase ATPase subunit family protein	-
SMATCC274_RS21095	4459462..4460148	+	687	WP_161545071.1	terminase family protein	-
SMATCC274_RS21100	4460145..4461179	+	1035	WP_033632040.1	phage portal protein	-
SMATCC274_RS21105	4461224..4461565	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SMATCC274_RS21110	4461565..4461819	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
SMATCC274_RS21115	4462280..4462543	-	264	WP_142076094.1	MazG-like family protein	-
SMATCC274_RS21120	4462691..4463017	+	327	WP_142076096.1	killer suppression protein HigA	-
SMATCC274_RS21125	4463014..4464096	+	1083	WP_142076098.1	ImmA/IrrE family metallo-endopeptidase	-
SMATCC274_RS21130	4464100..4465044	-	945	WP_142076100.1	hypothetical protein	-
SMATCC274_RS21135	4465041..4466021	-	981	WP_161545072.1	thymidylate synthase	-
SMATCC274_RS21140	4466294..4466518	-	225	WP_142076104.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	-	4425574..4496619	71045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T33408 WP_071605322.1 NZ_AP021873:c4461819-4461565 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T33408 NZ_AP021873:c4461819-4461565 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT33408 WP_033633573.1 NZ_AP021873:c4461565-4461224 [Serratia marcescens]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT33408 NZ_AP021873:c4461565-4461224 [Serratia marcescens]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1