Detailed information of TA system TA033384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2202239..2202779
• Replicon: chromosome
• Accession: NZ_AP021855
• Organism: Bradyrhizobium sp. TM102

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GDN70_RS10325
• Protein ID: WP_038973437.1
• Coordinates: 2202239..2202499 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A5H2YMS2
• Locus tag: GDN70_RS10330
• Protein ID: WP_028145613.1
• Coordinates: 2202486..2202779 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDN70_RS10305	2197727..2198560	+	834	WP_028145618.1	AraC family transcriptional regulator	-
GDN70_RS10310	2198699..2199637	+	939	WP_038973439.1	SMP-30/gluconolactonase/LRE family protein	-
GDN70_RS10315	2199796..2200389	+	594	WP_038950869.1	GNAT family N-acetyltransferase	-
GDN70_RS10320	2200406..2202052	+	1647	WP_038973438.1	GMC family oxidoreductase N-terminal domain-containing protein	-
GDN70_RS10325	2202239..2202499	+	261	WP_038973437.1	BrnT family toxin	Toxin
GDN70_RS10330	2202486..2202779	+	294	WP_028145613.1	BrnA antitoxin family protein	Antitoxin
GDN70_RS10340	2203784..2204626	-	843	WP_028145612.1	MoxR family ATPase	-
GDN70_RS10345	2204723..2204968	-	246	WP_018318587.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
GDN70_RS10350	2205092..2206267	+	1176	WP_028145611.1	VWA domain-containing protein	-
GDN70_RS10355	2206382..2206795	+	414	WP_028145610.1	rhodanese-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9784.33 Da
• Isoelectric Point: 10.3461

>T33384 WP_038973437.1 NZ_AP021855:2202239-2202499 [Bradyrhizobium sp. TM102]
MKIVWDEPKRLANFDEHGLDFADLNEAFFDTALVIPSHNKSKRWIAVGVSIRGVIVVVFARLGREGVSIISMRPASRSER
KLYAER

Nucleotide

• Length: 261 bp

>T33384 NZ_AP021855:2202239-2202499 [Bradyrhizobium sp. TM102]
ATGAAGATCGTCTGGGACGAACCGAAGCGGCTCGCCAACTTCGACGAGCACGGGCTGGACTTTGCCGATCTCAACGAAGC
GTTCTTCGACACCGCGCTGGTCATTCCCTCGCACAACAAGAGCAAGCGCTGGATCGCGGTCGGCGTGAGCATCCGTGGCG
TCATTGTTGTCGTATTCGCCCGGCTCGGCCGCGAGGGCGTCAGCATCATCAGCATGCGACCGGCAAGCCGCAGCGAAAGG
AAACTCTATGCCGAACGCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10799.50 Da
• Isoelectric Point: 11.3879

>AT33384 WP_028145613.1 NZ_AP021855:2202486-2202779 [Bradyrhizobium sp. TM102]
MPNARKKSGYTKRDLRAVSDNPELTKADFAKARPFAEVFPDLGASIRKGRGPNKAPTKKLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAP

Nucleotide

• Length: 294 bp

>AT33384 NZ_AP021855:2202486-2202779 [Bradyrhizobium sp. TM102]
ATGCCGAACGCTAGGAAGAAGTCAGGCTACACAAAGAGAGACCTGCGCGCAGTCAGCGATAATCCGGAGCTGACAAAGGC
GGACTTTGCCAAGGCGCGACCGTTTGCCGAGGTCTTCCCAGATCTCGGCGCATCCATCCGGAAGGGCCGCGGCCCGAACA
AGGCGCCGACGAAGAAGCTCGTCTCGTTGCGCCTCAGCCCGGAGGTGGTCGAGCATTTCAAGTCGACCGGGCCGGGCTGG
CAATCGCGGATCGACGAGACCTTGCGCAAGGCGGTCAAGCGCAAGGCGCCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5H2YMS2