33382

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 3095440..3095980 Replicon chromosome
Accession NZ_AP021854
Organism Bradyrhizobium sp. SG09

Toxin (Protein)


Gene name brnT Uniprot ID A0A5H2Z4C0
Locus tag GDN72_RS14920 Protein ID WP_028145614.1
Coordinates 3095440..3095700 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A5H2YMS2
Locus tag GDN72_RS14925 Protein ID WP_028145613.1
Coordinates 3095687..3095980 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GDN72_RS14900 3090933..3091766 + 834 WP_028145618.1 AraC family transcriptional regulator -
GDN72_RS14905 3091905..3092843 + 939 WP_028145617.1 SMP-30/gluconolactonase/LRE family protein -
GDN72_RS14910 3093002..3093595 + 594 WP_028145616.1 GNAT family N-acetyltransferase -
GDN72_RS14915 3093612..3095258 + 1647 WP_028145615.1 GMC family oxidoreductase N-terminal domain-containing protein -
GDN72_RS14920 3095440..3095700 + 261 WP_028145614.1 BrnT family toxin Toxin
GDN72_RS14925 3095687..3095980 + 294 WP_028145613.1 BrnA antitoxin family protein Antitoxin
GDN72_RS14930 3096984..3097826 - 843 WP_028145612.1 MoxR family ATPase -
GDN72_RS14935 3097922..3098167 - 246 WP_018318587.1 GlsB/YeaQ/YmgE family stress response membrane protein -
GDN72_RS14940 3098291..3099466 + 1176 WP_028145611.1 VWA domain-containing protein -
GDN72_RS14945 3099593..3100006 + 414 WP_028145610.1 rhodanese-like domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-79)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(20-92)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9783.39 Da        Isoelectric Point: 10.7978

>T33382 WP_028145614.1 NZ_AP021854:3095440-3095700 [Bradyrhizobium sp. SG09]
MKIVWDEPKRLANFDKHGLDFADLNEAFFDTALVIPSHNKSKRWIAVGVSIRGVIVVVFARLGREGVSIISMRPASRSER
KLYAER

Download         Length: 261 bp

>T33382 NZ_AP021854:3095440-3095700 [Bradyrhizobium sp. SG09]
ATGAAGATCGTCTGGGACGAACCGAAGCGGCTCGCCAACTTCGACAAGCACGGGCTGGACTTTGCCGATCTCAACGAAGC
GTTCTTCGACACCGCGCTGGTCATTCCCTCGCACAACAAGAGCAAGCGCTGGATCGCGGTCGGCGTGAGCATCCGTGGCG
TCATTGTTGTCGTATTCGCCCGGCTCGGCCGCGAGGGCGTCAGCATCATCAGCATGCGACCGGCAAGCCGCAGCGAAAGG
AAACTCTATGCCGAACGCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10799.50 Da        Isoelectric Point: 11.3879

>AT33382 WP_028145613.1 NZ_AP021854:3095687-3095980 [Bradyrhizobium sp. SG09]
MPNARKKSGYTKRDLRAVSDNPELTKADFAKARPFAEVFPDLGASIRKGRGPNKAPTKKLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAP

Download         Length: 294 bp

>AT33382 NZ_AP021854:3095687-3095980 [Bradyrhizobium sp. SG09]
ATGCCGAACGCTAGGAAGAAGTCAGGCTACACAAAGAGGGACCTGCGCGCAGTCAGCGATAATCCGGAGCTGACAAAGGC
GGACTTTGCCAAGGCGCGACCGTTTGCCGAGGTCTTCCCAGATCTCGGCGCATCCATCCGGAAGGGCCGCGGCCCGAACA
AGGCGCCGACGAAGAAGCTCGTCTCGTTGCGCCTCAGCCCGGAGGTGGTCGAGCATTTCAAGTCGACCGGGCCGGGCTGG
CAATCGCGGATCGACGAGACCTTGCGCAAGGCGGTCAAGCGCAAGGCGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5H2Z4C0

Antitoxin

Source ID Structure
AlphaFold DB A0A5H2YMS2

References