33374

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5396146..5396757 Replicon chromosome
Accession NZ_AP020337
Organism Pseudomonas sp. KUIN-1

Toxin (Protein)


Gene name PfiT Uniprot ID Q4ZME8
Locus tag KUIN1_RS24405 Protein ID WP_003304103.1
Coordinates 5396146..5396496 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q4ZME7
Locus tag KUIN1_RS24410 Protein ID WP_002556076.1
Coordinates 5396509..5396757 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUIN1_RS24385 5391820..5394306 + 2487 WP_024642071.1 bifunctional diguanylate cyclase/phosphodiesterase -
KUIN1_RS24395 5394489..5395496 + 1008 Protein_4496 P-type conjugative transfer protein TrbL -
KUIN1_RS24400 5395564..5395779 + 216 Protein_4497 hypothetical protein -
KUIN1_RS24405 5396146..5396496 - 351 WP_003304103.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KUIN1_RS24410 5396509..5396757 - 249 WP_002556076.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
KUIN1_RS24415 5397095..5398057 + 963 WP_103714631.1 tyrosine-type recombinase/integrase -
KUIN1_RS24420 5398083..5398397 - 315 WP_162884967.1 hypothetical protein -
KUIN1_RS24425 5398470..5398709 - 240 WP_011269146.1 hypothetical protein -
KUIN1_RS24430 5398789..5399235 - 447 WP_172980081.1 GNAT family N-acetyltransferase -
KUIN1_RS24435 5399291..5400034 - 744 Protein_4504 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5394585..5408257 13672
- flank IS/Tn - - 5399291..5399971 680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-98)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12973.76 Da        Isoelectric Point: 4.7690

>T33374 WP_003304103.1 NZ_AP020337:c5396496-5396146 [Pseudomonas sp. KUIN-1]
MNTFALRFTDVAQQSLEDQVEHLAVHQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSHQLSELGVLHYRELNTDGYRIFY
EVRDAGDINVIVVALVLGSKQSVEQALIRYCLLQAI

Download         Length: 351 bp

>T33374 NZ_AP020337:c5396496-5396146 [Pseudomonas sp. KUIN-1]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGTATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGTT
ACCCGGTCAGTCATCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGAGGGACGCAGGCGATATAAATGTCATCGTCGTGGCGCTCGTGTTAGGTAGCAAGCAAAGTGTTGAACAGGCCCT
CATCCGCTACTGCCTGCTTCAGGCTATATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9366.73 Da        Isoelectric Point: 9.9579

>AT33374 WP_002556076.1 NZ_AP020337:c5396757-5396509 [Pseudomonas sp. KUIN-1]
MRVETISFLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFT
QK

Download         Length: 249 bp

>AT33374 NZ_AP020337:c5396757-5396509 [Pseudomonas sp. KUIN-1]
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P9GDS9

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9GM89

References