Detailed information of TA system TA033373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 4434662..4435187
• Replicon: chromosome
• Accession: NZ_AP020337
• Organism: Pseudomonas sp. KUIN-1

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: Q4ZPQ9
• Locus tag: KUIN1_RS19845
• Protein ID: WP_011268681.1
• Coordinates: 4434897..4435187 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KUIN1_RS19840
• Protein ID: WP_152092216.1
• Coordinates: 4434662..4434907 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUIN1_RS19835	4430577..4434488	-	3912	WP_152092215.1	ATP-dependent RNA helicase HrpA	-
KUIN1_RS19840	4434662..4434907	+	246	WP_152092216.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KUIN1_RS19845	4434897..4435187	+	291	WP_011268681.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUIN1_RS19850	4435505..4437196	-	1692	WP_011268682.1	long-chain-fatty-acid--CoA ligase FadD1	-
KUIN1_RS19860	4437552..4439240	-	1689	WP_024640260.1	long-chain-fatty-acid--CoA ligase FadD2	-
KUIN1_RS19870	4439609..4440079	+	471	WP_003402365.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11010.01 Da
• Isoelectric Point: 10.7294

>T33373 WP_011268681.1 NZ_AP020337:4434897-4435187 [Pseudomonas sp. KUIN-1]
MTFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGYRLVYRVEEDRVVVTVVAVGK
RERGNIYDSAKGRLGP

Nucleotide

• Length: 291 bp

>T33373 NZ_AP020337:4434897-4435187 [Pseudomonas sp. KUIN-1]
ATGACCTTTAAGCTTGAATTTCTGCCCTCGGCCTTGAAGGAGTGGGAAAAACTGGGCCATACCATCCGTGTTCAGCTCAA
GAAAAAGCTTCTCGAACGACTCGGACTACCAAGAATTCCAGGCGATGCGCTTCATGGCATGCCAGATCATTACAAAATCA
AACTGCGCAGTGCAGGATACCGACTCGTCTATCGAGTCGAGGAAGATAGGGTAGTGGTAACCGTCGTTGCGGTAGGCAAG
CGCGAGCGCGGGAATATTTATGATTCGGCCAAAGGCAGGCTTGGCCCTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8896.33 Da
• Isoelectric Point: 4.7440

>AT33373 WP_152092216.1 NZ_AP020337:4434662-4434907 [Pseudomonas sp. KUIN-1]
MQRILADVAVSVSELKKNPSAVLSGAHGLPVAVLNHNKVMGYMVPAEVFEAMMERLDDLDLAELIKSRQHEKPVSVDIDD
L

Nucleotide

• Length: 246 bp

>AT33373 NZ_AP020337:4434662-4434907 [Pseudomonas sp. KUIN-1]
ATGCAACGCATCCTTGCAGATGTCGCGGTCAGCGTCTCTGAACTCAAGAAAAATCCGTCAGCGGTCCTGAGTGGCGCTCA
TGGTTTACCTGTGGCCGTCCTTAATCACAATAAAGTCATGGGCTACATGGTCCCTGCCGAGGTGTTTGAAGCAATGATGG
AGAGGCTTGATGACCTTGATCTGGCCGAGCTGATCAAATCTCGCCAACATGAAAAGCCGGTGTCGGTAGATATAGATGAC
CTTTAA

Structures

Toxin

• AlphaFold DB: Q4ZPQ9