Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 20968..21595 Replicon plasmid p2-JPH1
Accession NZ_AP020328
Organism Mycobacterium avium subsp. hominissuis strain JP-H-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A854IFX7
Locus tag JPH1_RS27020 Protein ID WP_054585721.1
Coordinates 20968..21366 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U5MRR7
Locus tag JPH1_RS27025 Protein ID WP_054585712.1
Coordinates 21359..21595 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JPH1_RS26995 16554..17003 + 450 WP_054585713.1 hypothetical protein -
JPH1_RS27000 17119..17865 - 747 WP_082385902.1 Crp/Fnr family transcriptional regulator -
JPH1_RS27005 18156..18419 - 264 WP_139315510.1 hypothetical protein -
JPH1_RS27010 18518..19921 - 1404 WP_054585723.1 DUF2156 domain-containing protein -
JPH1_RS27015 20140..20718 - 579 WP_054585722.1 recombinase family protein -
JPH1_RS27020 20968..21366 - 399 WP_054585721.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JPH1_RS27025 21359..21595 - 237 WP_054585712.1 antitoxin Antitoxin
JPH1_RS27030 21725..23209 - 1485 WP_054585711.1 NAD(P)/FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..39206 39206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14306.44 Da        Isoelectric Point: 6.9865

>T33367 WP_054585721.1 NZ_AP020328:c21366-20968 [Mycobacterium avium subsp. hominissuis]
MPEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPAVSPAEGVDTDSVAVCRSI
RAVARTRLVERLGTIRPGTMREIEDVLTLILGIYTAQDRGEPTLRRPFLSDT

Download         Length: 399 bp

>T33367 NZ_AP020328:c21366-20968 [Mycobacterium avium subsp. hominissuis]
TTGCCTGAGCCGCGACGGGGCGACCTCTGGCTGGTCAGCCTTGGCGCCGCTCGCGCCGGTGAGCCCGGCAAACATCGCCC
CGCCGTAGTCGTCTCCGTCGATGAGCTACTCACCGGCATCGACGACGAACTCGTCGTGGTCGTGCCAGTGTCAAGCTCCC
GCTCGCGCACCCCGCTCCGGCCAGCCGTCTCGCCCGCTGAAGGTGTGGATACTGATAGCGTTGCCGTCTGCCGCAGCATC
CGCGCAGTCGCCCGCACCCGCCTAGTGGAACGACTCGGCACCATCAGGCCCGGCACGATGCGCGAAATCGAAGATGTCCT
GACCCTCATTCTCGGCATCTACACGGCACAAGACCGTGGCGAGCCGACATTACGGCGCCCCTTCCTGAGTGACACATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8636.54 Da        Isoelectric Point: 4.8047

>AT33367 WP_054585712.1 NZ_AP020328:c21595-21359 [Mycobacterium avium subsp. hominissuis]
MSTSTTTIRVPIQTRDRLAAQARERGISISAFLTELASQAERQAIFRVERDAIHAEAAAHAVREEDRDWEDTVGDGLA

Download         Length: 237 bp

>AT33367 NZ_AP020328:c21595-21359 [Mycobacterium avium subsp. hominissuis]
ATGTCTACATCCACGACGACGATCAGGGTTCCCATCCAGACGCGTGACCGCCTAGCCGCCCAAGCCCGTGAACGAGGAAT
CTCGATCTCCGCTTTCCTCACCGAACTGGCCTCCCAGGCCGAGCGCCAGGCGATCTTCCGCGTCGAACGCGACGCCATAC
ACGCCGAAGCGGCTGCCCACGCCGTGCGCGAGGAGGACCGCGATTGGGAGGACACCGTAGGCGATGGCCTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854IFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U5MRR7

References