Detailed information of TA system TA033364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1467929..1468493
• Replicon: chromosome
• Accession: NZ_AP020326
• Organism: Mycobacterium avium subsp. hominissuis strain JP-H-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: JPH1_RS07275
• Protein ID: WP_003875391.1
• Coordinates: 1468122..1468493 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JPH1_RS07270
• Protein ID: WP_011724021.1
• Coordinates: 1467929..1468135 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JPH1_RS07255	1464118..1465191	+	1074	WP_033710961.1	redox-regulated ATPase YchF	-
JPH1_RS07260	1465391..1466335	+	945	WP_003875389.1	HNH endonuclease	-
JPH1_RS07265	1466515..1467750	-	1236	WP_151834828.1	IS256-like element IS1311 family transposase	-
JPH1_RS07270	1467929..1468135	+	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JPH1_RS07275	1468122..1468493	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
JPH1_RS07280	1468593..1469033	+	441	WP_003875392.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
JPH1_RS07285	1469099..1469836	-	738	WP_011724023.1	glucose 1-dehydrogenase	-
JPH1_RS07290	1469932..1470537	+	606	WP_011724024.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
JPH1_RS07295	1470639..1471037	+	399	WP_011724025.1	VOC family protein	-
JPH1_RS07300	1471106..1471489	+	384	WP_011724026.1	hypothetical protein	-
JPH1_RS07305	1471559..1471891	+	333	WP_011724027.1	hypothetical protein	-
JPH1_RS07310	1472048..1472596	+	549	WP_080516335.1	glycoside hydrolase	-
JPH1_RS07315	1473007..1473330	+	324	WP_044543559.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1466515..1467750	1235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T33364 WP_003875391.1 NZ_AP020326:1468122-1468493 [Mycobacterium avium subsp. hominissuis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T33364 NZ_AP020326:1468122-1468493 [Mycobacterium avium subsp. hominissuis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGCTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTTGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT33364 WP_011724021.1 NZ_AP020326:1467929-1468135 [Mycobacterium avium subsp. hominissuis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT33364 NZ_AP020326:1467929-1468135 [Mycobacterium avium subsp. hominissuis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGACACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37