Detailed information of TA system TA033363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1236792..1237445
• Replicon: chromosome
• Accession: NZ_AP020326
• Organism: Mycobacterium avium subsp. hominissuis strain JP-H-1

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JPH1_RS06185
• Protein ID: WP_003872880.1
• Coordinates: 1236984..1237445 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: JPH1_RS06180
• Protein ID: WP_003872881.1
• Coordinates: 1236792..1236980 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JPH1_RS06170	1232774..1234360	+	1587	WP_023900196.1	serine hydrolase	-
JPH1_RS06175	1234357..1236729	+	2373	WP_088295086.1	cation-translocating P-type ATPase	-
JPH1_RS06180	1236792..1236980	+	189	WP_003872881.1	antitoxin	Antitoxin
JPH1_RS06185	1236984..1237445	+	462	WP_003872880.1	SRPBCC family protein	Toxin
JPH1_RS06190	1237515..1237949	+	435	WP_023880080.1	hypothetical protein	-
JPH1_RS06195	1238948..1240531	+	1584	WP_003872878.1	DUF4185 domain-containing protein	-
JPH1_RS06200	1240578..1240959	+	382	Protein_1212	hypothetical protein	-
JPH1_RS06210	1241751..1242089	+	339	WP_009975166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T33363 WP_003872880.1 NZ_AP020326:1236984-1237445 [Mycobacterium avium subsp. hominissuis]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T33363 NZ_AP020326:1236984-1237445 [Mycobacterium avium subsp. hominissuis]
GTGGCCAAGCTTTCCGGATCCATCGATGTTCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGCATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGCTCGGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCAATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT33363 WP_003872881.1 NZ_AP020326:1236792-1236980 [Mycobacterium avium subsp. hominissuis]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT33363 NZ_AP020326:1236792-1236980 [Mycobacterium avium subsp. hominissuis]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18