Detailed information of TA system TA033361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 956584..957251
• Replicon: chromosome
• Accession: NZ_AP020326
• Organism: Mycobacterium avium subsp. hominissuis strain JP-H-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D5PE24
• Locus tag: JPH1_RS04710
• Protein ID: WP_007168407.1
• Coordinates: 956859..957251 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D5PE23
• Locus tag: JPH1_RS04705
• Protein ID: WP_007168406.1
• Coordinates: 956584..956859 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JPH1_RS27265	952088..952414	-	327	WP_033711234.1	hypothetical protein	-
JPH1_RS04675	952404..952637	-	234	Protein_912	transposase	-
JPH1_RS04680	952655..953866	-	1212	Protein_913	ISL3 family transposase	-
JPH1_RS04685	954005..954637	-	633	Protein_914	DDE-type integrase/transposase/recombinase	-
JPH1_RS27560	954589..954729	-	141	WP_145958858.1	SDR family oxidoreductase	-
JPH1_RS04695	954773..955476	-	704	Protein_916	histidine phosphatase family protein	-
JPH1_RS04700	955559..956431	+	873	WP_007168405.1	MaoC family dehydratase N-terminal domain-containing protein	-
JPH1_RS04705	956584..956859	+	276	WP_007168406.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JPH1_RS04710	956859..957251	+	393	WP_007168407.1	type II toxin-antitoxin system VapC family toxin	Toxin
JPH1_RS04715	957399..958558	-	1160	Protein_920	trehalose-phosphatase	-
JPH1_RS04720	958600..959938	-	1339	Protein_921	DUF1298 domain-containing protein	-
JPH1_RS04725	960199..960321	-	123	Protein_922	IS1595 family transposase	-
JPH1_RS04730	960658..961992	+	1335	Protein_923	IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-45)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14305.73 Da
• Isoelectric Point: 10.5032

>T33361 WP_007168407.1 NZ_AP020326:956859-957251 [Mycobacterium avium subsp. hominissuis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRLPRVSSALARTEVMRALLHKGESARRAGRRALASLDLLRIDNRLLDLAG
GLLPIELRTLDAIHLATAQRLGMDLGRLCTYDDRMRDAAEALGMAVIAPS

Nucleotide

• Length: 393 bp

>T33361 NZ_AP020326:956859-957251 [Mycobacterium avium subsp. hominissuis]
ATGGCGGCCATCTACCTGGACTCGTCCGCAATCGTCAAGCTTGCAGTTCGTGAGCCAGAGTCGGATGCGCTGCGTCGCTA
CCTTCGCACCCGGCTGCCGCGGGTGTCGAGTGCCCTTGCGCGGACGGAGGTCATGCGGGCTCTGCTCCACAAGGGTGAAT
CAGCCCGAAGAGCCGGACGCCGGGCGCTCGCGAGCCTAGACCTACTACGCATCGACAACCGATTGCTCGATCTTGCTGGT
GGGCTGCTGCCAATCGAGCTACGCACCCTCGATGCAATTCACCTGGCGACTGCCCAGCGGCTCGGCATGGATCTTGGCCG
GTTGTGCACTTACGACGACCGCATGCGCGACGCCGCCGAGGCGCTCGGCATGGCAGTGATTGCCCCGTCTTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10150.63 Da
• Isoelectric Point: 5.1881

>AT33361 WP_007168406.1 NZ_AP020326:956584-956859 [Mycobacterium avium subsp. hominissuis]
MSTVGVRELRQRASELLRRVEAGETIEITDRGRPIALLSPMPEGGPYQQMLASGGIERATLDFDDLPEPVRLKTGIELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT33361 NZ_AP020326:956584-956859 [Mycobacterium avium subsp. hominissuis]
ATGTCTACGGTGGGCGTTCGTGAGCTGCGGCAGCGGGCCAGCGAACTCCTCCGTCGGGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGTGGTCGGCCTATCGCCCTTCTCTCGCCGATGCCGGAGGGCGGTCCGTACCAGCAGATGCTGGCAAGCG
GCGGGATCGAACGTGCGACGCTTGACTTTGATGACCTTCCCGAGCCGGTCCGACTCAAAACGGGTATCGAGCTGCCGTCC
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A1X1XV84

Antitoxin

• AlphaFold DB: A0A1X1XUZ3