Detailed information of TA system TA033359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2824034..2824563
• Replicon: chromosome
• Accession: NZ_AP020324
• Organism: Staphylococcus aureus strain KUN1163

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KUH140013_RS14370
• Protein ID: WP_000621175.1
• Coordinates: 2824201..2824563 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KUH140013_RS14365
• Protein ID: WP_000948331.1
• Coordinates: 2824034..2824204 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUH140013_RS14335	2819071..2819631	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KUH140013_RS14340	2819839..2820318	+	480	WP_001287087.1	hypothetical protein	-
KUH140013_RS14345	2820311..2821894	+	1584	WP_001294626.1	PH domain-containing protein	-
KUH140013_RS14350	2821881..2822372	+	492	WP_001205912.1	PH domain-containing protein	-
KUH140013_RS14355	2822376..2822735	+	360	WP_000581197.1	holo-ACP synthase	-
KUH140013_RS14360	2822801..2823949	+	1149	WP_001281154.1	alanine racemase	-
KUH140013_RS14365	2824034..2824204	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KUH140013_RS14370	2824201..2824563	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KUH140013_RS14375	2824912..2825913	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KUH140013_RS14380	2826032..2826358	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KUH140013_RS14385	2826360..2826839	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KUH140013_RS14390	2826814..2827584	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T33359 WP_000621175.1 NZ_AP020324:2824201-2824563 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T33359 NZ_AP020324:2824201-2824563 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT33359 WP_000948331.1 NZ_AP020324:2824034-2824204 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT33359 NZ_AP020324:2824034-2824204 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1