Detailed information of TA system TA033345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 506583..507371
• Replicon: chromosome
• Accession: NZ_AP020324
• Organism: Staphylococcus aureus strain KUN1163

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2I7Y5B3
• Locus tag: KUH140013_RS02760
• Protein ID: WP_000525004.1
• Coordinates: 506583..507044 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KUH140013_RS02765
• Protein ID: WP_000333631.1
• Coordinates: 507057..507371 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUH140013_RS02735	501593..503620	+	2028	WP_001558502.1	hypothetical protein	-
KUH140013_RS02740	503663..504868	-	1206	WP_000264751.1	site-specific integrase	-
KUH140013_RS02745	504994..505608	+	615	WP_000191470.1	hypothetical protein	-
KUH140013_RS02750	505605..505751	-	147	WP_001795334.1	hypothetical protein	-
KUH140013_RS02755	505981..506565	-	585	WP_000825949.1	hypothetical protein	-
KUH140013_RS02760	506583..507044	-	462	WP_000525004.1	hypothetical protein	Toxin
KUH140013_RS02765	507057..507371	-	315	WP_000333631.1	helix-turn-helix domain-containing protein	Antitoxin
KUH140013_RS02770	507523..507759	+	237	WP_001121027.1	helix-turn-helix domain-containing protein	-
KUH140013_RS02775	507773..508549	+	777	WP_001148544.1	Rha family transcriptional regulator	-
KUH140013_RS02780	508578..508823	+	246	WP_001025401.1	DUF2829 domain-containing protein	-
KUH140013_RS02785	508792..509157	-	366	WP_001128433.1	hypothetical protein	-
KUH140013_RS02790	509212..509427	+	216	WP_001097552.1	hypothetical protein	-
KUH140013_RS02795	509454..509717	+	264	WP_001124194.1	helix-turn-helix domain-containing protein	-
KUH140013_RS02800	509730..509891	+	162	WP_001285948.1	DUF1270 domain-containing protein	-
KUH140013_RS02805	509970..510293	+	324	WP_000174999.1	hypothetical protein	-
KUH140013_RS02810	510308..510670	+	363	WP_000985972.1	hypothetical protein	-
KUH140013_RS02815	510667..511833	+	1167	WP_000762552.1	DUF2800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18074.46 Da
• Isoelectric Point: 4.6915

>T33345 WP_000525004.1 NZ_AP020324:c507044-506583 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T33345 NZ_AP020324:c507044-506583 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12062.58 Da
• Isoelectric Point: 7.1661

>AT33345 WP_000333631.1 NZ_AP020324:c507371-507057 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYANYIRSKRK

Nucleotide

• Length: 315 bp

>AT33345 NZ_AP020324:c507371-507057 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCTAATTATATAAGAAGCAAACGTAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2I7Y5B3