Detailed information of TA system TA033338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2838809..2839287
• Replicon: chromosome
• Accession: NZ_AP020322
• Organism: Staphylococcus aureus strain KUH180129

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: A0A0H3K0C1
• Locus tag: F6Y14_RS14460
• Protein ID: WP_001078344.1
• Coordinates: 2838961..2839287 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: F6Y14_RS14455
• Protein ID: WP_025175789.1
• Coordinates: 2838809..2838958 (+)
• Length: 50 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y14_RS14420	2833995..2834279	+	285	WP_000917289.1	co-chaperone GroES	-
F6Y14_RS14425	2834355..2835971	+	1617	WP_000240642.1	chaperonin GroEL	-
F6Y14_RS14430	2836040..2837212	-	1173	WP_000179343.1	site-specific integrase	-
F6Y14_RS14435	2837226..2837900	-	675	WP_000620857.1	helix-turn-helix transcriptional regulator	-
F6Y14_RS14440	2838073..2838291	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
F6Y14_RS14445	2838296..2838613	+	318	WP_000481967.1	helix-turn-helix domain-containing protein	-
F6Y14_RS14450	2838610..2838756	+	147	WP_000784885.1	hypothetical protein	-
F6Y14_RS14455	2838809..2838958	+	150	WP_025175789.1	hypothetical protein	Antitoxin
F6Y14_RS14460	2838961..2839287	+	327	WP_001078344.1	DUF1474 family protein	Toxin
F6Y14_RS14465	2839352..2840221	+	870	WP_001002689.1	primase alpha helix C-terminal domain-containing protein	-
F6Y14_RS14470	2840235..2841944	+	1710	WP_000447473.1	DUF927 domain-containing protein	-
F6Y14_RS14475	2842254..2842634	+	381	WP_000356942.1	hypothetical protein	-
F6Y14_RS14480	2842631..2843272	+	642	WP_001047698.1	hypothetical protein	-
F6Y14_RS14485	2843722..2844063	+	342	WP_001190615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL / tsst-1 / sec / sell / hlb	2833995..2876264	42269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12968.26 Da
• Isoelectric Point: 4.3050

>T33338 WP_001078344.1 NZ_AP020322:2838961-2839287 [Staphylococcus aureus]
MNREIKDLFSDLKLLKDSFEDLKDSNGWHFDELYPYEPNHVLNKDELIGEGFSYHERRIHNNQMFDLFHLYIEQFDNIIE
KFYEIEKASSENFGEESDDAKNSIKVAE

Nucleotide

• Length: 327 bp

>T33338 NZ_AP020322:2838961-2839287 [Staphylococcus aureus]
ATGAATCGGGAAATTAAAGATTTATTTAGCGATTTAAAGTTGTTGAAAGATAGTTTTGAGGATTTAAAGGATAGTAATGG
TTGGCATTTTGATGAGCTATATCCATATGAACCAAATCATGTCTTAAATAAAGATGAATTAATTGGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTTATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin

Protein

• Length: 50 a.a.
• Molecular weight: 5822.52 Da
• Isoelectric Point: 4.4876

>AT33338 WP_025175789.1 NZ_AP020322:2838809-2838958 [Staphylococcus aureus]
VNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 150 bp

>AT33338 NZ_AP020322:2838809-2838958 [Staphylococcus aureus]
GTGAATAATTTGGAAAGTTATGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCA
CTTAGAATTGATAATGAAATGGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3K0C1