Detailed information of TA system TA033337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2786869..2787398
• Replicon: chromosome
• Accession: NZ_AP020322
• Organism: Staphylococcus aureus strain KUH180129

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F6Y14_RS14190
• Protein ID: WP_000621175.1
• Coordinates: 2787036..2787398 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: F6Y14_RS14185
• Protein ID: WP_000948331.1
• Coordinates: 2786869..2787039 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y14_RS14155	2781906..2782466	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
F6Y14_RS14160	2782674..2783153	+	480	WP_001287087.1	hypothetical protein	-
F6Y14_RS14165	2783146..2784729	+	1584	WP_150073953.1	PH domain-containing protein	-
F6Y14_RS14170	2784716..2785207	+	492	WP_001205912.1	PH domain-containing protein	-
F6Y14_RS14175	2785211..2785570	+	360	WP_000581197.1	holo-ACP synthase	-
F6Y14_RS14180	2785636..2786784	+	1149	WP_001281154.1	alanine racemase	-
F6Y14_RS14185	2786869..2787039	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
F6Y14_RS14190	2787036..2787398	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6Y14_RS14195	2787747..2788748	+	1002	WP_086031886.1	PP2C family protein-serine/threonine phosphatase	-
F6Y14_RS14200	2788867..2789193	+	327	WP_001052491.1	anti-sigma factor antagonist	-
F6Y14_RS14205	2789195..2789674	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
F6Y14_RS14210	2789649..2790419	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T33337 WP_000621175.1 NZ_AP020322:2787036-2787398 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T33337 NZ_AP020322:2787036-2787398 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT33337 WP_000948331.1 NZ_AP020322:2786869-2787039 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT33337 NZ_AP020322:2786869-2787039 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1