Detailed information of TA system TA033306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1631335..1631864
• Replicon: chromosome
• Accession: NZ_AP020320
• Organism: Staphylococcus aureus strain KUH180062

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F6Y17_RS08455
• Protein ID: WP_000621175.1
• Coordinates: 1631335..1631697 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: F6Y17_RS08460
• Protein ID: WP_000948331.1
• Coordinates: 1631694..1631864 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y17_RS08435	1628314..1629084	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
F6Y17_RS08440	1629059..1629538	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
F6Y17_RS08445	1629540..1629866	-	327	WP_001052491.1	anti-sigma factor antagonist	-
F6Y17_RS08450	1629985..1630986	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
F6Y17_RS08455	1631335..1631697	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6Y17_RS08460	1631694..1631864	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
F6Y17_RS08465	1631949..1633097	-	1149	WP_001281154.1	alanine racemase	-
F6Y17_RS08470	1633163..1633522	-	360	WP_000581197.1	holo-ACP synthase	-
F6Y17_RS08475	1633526..1634017	-	492	WP_001205912.1	PH domain-containing protein	-
F6Y17_RS08480	1634004..1635587	-	1584	WP_001294626.1	PH domain-containing protein	-
F6Y17_RS08485	1635580..1636059	-	480	WP_001287087.1	hypothetical protein	-
F6Y17_RS08490	1636267..1636827	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T33306 WP_000621175.1 NZ_AP020320:c1631697-1631335 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T33306 NZ_AP020320:c1631697-1631335 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT33306 WP_000948331.1 NZ_AP020320:c1631864-1631694 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT33306 NZ_AP020320:c1631864-1631694 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1