Detailed information of TA system TA033301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 505096..505884
• Replicon: chromosome
• Accession: NZ_AP020320
• Organism: Staphylococcus aureus strain KUH180062

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2I7Y5B3
• Locus tag: F6Y17_RS02750
• Protein ID: WP_000525004.1
• Coordinates: 505096..505557 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F6Y17_RS02755
• Protein ID: WP_000333631.1
• Coordinates: 505570..505884 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y17_RS02725	500106..502133	+	2028	WP_001558502.1	hypothetical protein	-
F6Y17_RS02730	502176..503381	-	1206	WP_000264751.1	site-specific integrase	-
F6Y17_RS02735	503507..504121	+	615	WP_000191470.1	hypothetical protein	-
F6Y17_RS02740	504118..504264	-	147	WP_001795334.1	hypothetical protein	-
F6Y17_RS02745	504494..505078	-	585	WP_000825949.1	hypothetical protein	-
F6Y17_RS02750	505096..505557	-	462	WP_000525004.1	hypothetical protein	Toxin
F6Y17_RS02755	505570..505884	-	315	WP_000333631.1	helix-turn-helix domain-containing protein	Antitoxin
F6Y17_RS02760	506036..506272	+	237	WP_001121027.1	helix-turn-helix domain-containing protein	-
F6Y17_RS02765	506286..507062	+	777	WP_001148544.1	Rha family transcriptional regulator	-
F6Y17_RS02770	507091..507336	+	246	WP_001025401.1	DUF2829 domain-containing protein	-
F6Y17_RS02775	507305..507670	-	366	WP_001128433.1	hypothetical protein	-
F6Y17_RS02780	507725..507940	+	216	WP_001097552.1	hypothetical protein	-
F6Y17_RS02785	507967..508230	+	264	WP_001124194.1	helix-turn-helix domain-containing protein	-
F6Y17_RS02790	508243..508404	+	162	WP_001285948.1	DUF1270 domain-containing protein	-
F6Y17_RS02795	508483..508806	+	324	WP_000174999.1	hypothetical protein	-
F6Y17_RS02800	508821..509183	+	363	WP_000985972.1	hypothetical protein	-
F6Y17_RS02805	509180..510346	+	1167	WP_000762552.1	DUF2800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18074.46 Da
• Isoelectric Point: 4.6915

>T33301 WP_000525004.1 NZ_AP020320:c505557-505096 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T33301 NZ_AP020320:c505557-505096 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12062.58 Da
• Isoelectric Point: 7.1661

>AT33301 WP_000333631.1 NZ_AP020320:c505884-505570 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYANYIRSKRK

Nucleotide

• Length: 315 bp

>AT33301 NZ_AP020320:c505884-505570 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCTAATTATATAAGAAGCAAACGTAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2I7Y5B3