Detailed information of TA system TA033275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2673822..2674351
• Replicon: chromosome
• Accession: NZ_AP020316
• Organism: Staphylococcus aureus strain KUH140331

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F6Y18_RS13415
• Protein ID: WP_000621175.1
• Coordinates: 2673989..2674351 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: F6Y18_RS13410
• Protein ID: WP_000948331.1
• Coordinates: 2673822..2673992 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y18_RS13380	2668858..2669418	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
F6Y18_RS13385	2669627..2670106	+	480	WP_001287079.1	hypothetical protein	-
F6Y18_RS13390	2670099..2671682	+	1584	WP_001294620.1	PH domain-containing protein	-
F6Y18_RS13395	2671669..2672160	+	492	WP_001205912.1	PH domain-containing protein	-
F6Y18_RS13400	2672164..2672523	+	360	WP_000581197.1	holo-ACP synthase	-
F6Y18_RS13405	2672589..2673737	+	1149	WP_150030517.1	alanine racemase	-
F6Y18_RS13410	2673822..2673992	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
F6Y18_RS13415	2673989..2674351	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6Y18_RS13420	2674700..2675701	+	1002	WP_150030518.1	PP2C family protein-serine/threonine phosphatase	-
F6Y18_RS13425	2675820..2676146	+	327	WP_001052491.1	anti-sigma factor antagonist	-
F6Y18_RS13430	2676148..2676627	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
F6Y18_RS13435	2676602..2677372	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T33275 WP_000621175.1 NZ_AP020316:2673989-2674351 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T33275 NZ_AP020316:2673989-2674351 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT33275 WP_000948331.1 NZ_AP020316:2673822-2673992 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT33275 NZ_AP020316:2673822-2673992 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1