Detailed information of TA system TA033274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2654079..2654593
• Replicon: chromosome
• Accession: NZ_AP020316
• Organism: Staphylococcus aureus strain KUH140331

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: F6Y18_RS13325
• Protein ID: WP_000581792.1
• Coordinates: 2654384..2654593 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: F6Y18_RS13320
• Protein ID: WP_000138932.1
• Coordinates: 2654079..2654372 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y18_RS13285	2649443..2650084	+	642	WP_000483156.1	thiamine phosphate synthase	-
F6Y18_RS13290	2650170..2651042	+	873	WP_000725800.1	membrane protein insertase YidC	-
F6Y18_RS13310	2651754..2652401	-	648	WP_001187630.1	HD domain-containing protein	-
F6Y18_RS13315	2652429..2653913	-	1485	WP_000571549.1	cardiolipin synthase	-
F6Y18_RS13320	2654079..2654372	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
F6Y18_RS13325	2654384..2654593	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
F6Y18_RS13330	2654721..2654858	+	138	WP_000828354.1	hypothetical protein	-
F6Y18_RS13335	2655122..2656324	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
F6Y18_RS13340	2656642..2657712	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
F6Y18_RS13345	2657727..2659085	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T33274 WP_000581792.1 NZ_AP020316:2654384-2654593 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T33274 NZ_AP020316:2654384-2654593 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT33274 WP_000138932.1 NZ_AP020316:2654079-2654372 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT33274 NZ_AP020316:2654079-2654372 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13